Western blot abreview for Anti-RPS16 antibody

Good
Abreviews
Application
Western blot
Sample
Chinese Hamster Cell lysate - whole cell (CHO DG44)
Loading amount
100000 cells
Specification
CHO DG44
Gel Running Conditions
Reduced Denaturing (12.5%)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C

Other product details

Dilution
1/1000
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% BSA in TBST buffer

Secondary antibody

Name
Non-Abcam antibody was used: Goat anti-rabbit-HRP (cs7074)
Host species: Goat
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/10000

Detection

Detection method
chemiluminescence
Exposure
3 minute(s) and 0 second(s)
Bands
Specific: 16 kDa
Positive control
ß-actin (~ 42 kDa) primary antibody (Sigma A5441)1/10000 diluted secondary antibody (Sigma A2554)1/10000 diluted

Mr. Sven Mathias

Verified customer

Submitted Aug 27 2012

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