RecombinantRabMAb

Recombinant Anti-RPS16 antibody [EPR11755] (ab177951)

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Immunoprecipitation - Anti-RPS16 antibody [EPR11755] (ab177951)
  • Immunocytochemistry/ Immunofluorescence - Anti-RPS16 antibody [EPR11755] (ab177951)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS16 antibody [EPR11755] (ab177951)
  • Western blot - Anti-RPS16 antibody [EPR11755] (ab177951)
  • Anti-RPS16 antibody [EPR11755] (ab177951)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR11755] to RPS16
  • Suitable for: ICC/IF, IP, IHC-P, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-RPS16 antibody [EPR11755]
    See all RPS16 primary antibodies

  • Description

    Rabbit monoclonal [EPR11755] to RPS16

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC/IF, IP, IHC-P, WBmore details
    Unsuitable for: Flow Cyt

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human RPS16 aa 100 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
    Database link: P62249

  • Positive control

    • MCF-7, fetal liver, HeLa, Ramos, PC-12 and NIH/3T3 cell lysates; Breast carcinoma tissue; MCF-7 cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR11755

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab177951 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100 - 1/250.
IP 1/10 - 1/100.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/1000 - 1/5000. Predicted molecular weight: 16 kDa.
  • Application notes
    Is unsuitable for Flow Cyt.

    • Target

    • Relevance

      Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. RPS16 is a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S9P family of ribosomal proteins. It is located in the cytoplasm. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.

    • Database links

      see all

    • Alternative names

      • 40S ribosomal protein S16 antibody
      • Ribosomal protein S16 antibody
      • RPS 16 antibody
      • S16 antibody

    Images

    • Immunoprecipitation - Anti-RPS16 antibody [EPR11755] (ab177951)
      Immunoprecipitation - Anti-RPS16 antibody [EPR11755] (ab177951)

      Western blot analysis on immunoprecipitation pellet from fetal liver lysate using ab177951.

    • Immunocytochemistry/ Immunofluorescence - Anti-RPS16 antibody [EPR11755] (ab177951)
      Immunocytochemistry/ Immunofluorescence - Anti-RPS16 antibody [EPR11755] (ab177951)

      Immunofluorescent analysis of MCF-7 cells labeling RPS16 with ab177951 at 1/100 dilution.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS16 antibody [EPR11755] (ab177951)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS16 antibody [EPR11755] (ab177951)

      Immunohistochemical analysis of paraffin-embedded breast carcinoma tissue labeling RPS16 with ab177951 at 1/50 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Western blot - Anti-RPS16 antibody [EPR11755] (ab177951)
      Western blot - Anti-RPS16 antibody [EPR11755] (ab177951)
      All lanes : Anti-RPS16 antibody [EPR11755] (ab177951) at 1/1000 dilution

      Lane 1 : MCF-7 cell lysate
      Lane 2 : Fetal liver lysate
      Lane 3 : HeLa cell lysate
      Lane 4 : Ramos cell lysate
      Lane 5 : PC-12 cell lysate
      Lane 6 : NIH/3T3 cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 16 kDa

    • Anti-RPS16 antibody [EPR11755] (ab177951)
      Anti-RPS16 antibody [EPR11755] (ab177951)

    References (0)

    Publishing research using ab177951? Please let us know so that we can cite the reference in this datasheet.

    ab177951 has not yet been referenced specifically in any publications.

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