Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11755] to RPS16
- Suitable for: ICC/IF, IP, IHC-P, WB
- Reacts with: Mouse, Rat, Human
Product nameAnti-RPS16 antibody [EPR11755]
See all RPS16 primary antibodies
DescriptionRabbit monoclonal [EPR11755] to RPS16
Tested applicationsSuitable for: ICC/IF, IP, IHC-P, WBmore details
Unsuitable for: Flow Cyt
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human RPS16 aa 100 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
Database link: P62249
- MCF-7, fetal liver, HeLa, Ramos, PC-12 and NIH/3T3 cell lysates; Breast carcinoma tissue; MCF-7 cells.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab177951 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/100 - 1/250.|
|IP||1/10 - 1/100.|
|IHC-P||1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||1/1000 - 1/5000. Predicted molecular weight: 16 kDa.|
RelevanceRibosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. RPS16 is a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S9P family of ribosomal proteins. It is located in the cytoplasm. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.
- 40S ribosomal protein S16 antibody
- Ribosomal protein S16 antibody
- RPS 16 antibody
- S16 antibody
Western blot analysis on immunoprecipitation pellet from fetal liver lysate using ab177951.
Immunofluorescent analysis of MCF-7 cells labeling RPS16 with ab177951 at 1/100 dilution.
Immunohistochemical analysis of paraffin-embedded breast carcinoma tissue labeling RPS16 with ab177951 at 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
All lanes : Anti-RPS16 antibody [EPR11755] (ab177951) at 1/1000 dilution
Lane 1 : MCF-7 cell lysate
Lane 2 : Fetal liver lysate
Lane 3 : HeLa cell lysate
Lane 4 : Ramos cell lysate
Lane 5 : PC-12 cell lysate
Lane 6 : NIH/3T3 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 16 kDa
ab177951 has not yet been referenced specifically in any publications.