Recombinant
RabMAb

Recombinant Anti-RPS16 antibody [EPR11755] (ab177951)

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Overview

  • Product name

    Anti-RPS16 antibody [EPR11755]
    See all RPS16 primary antibodies

  • Description

    Rabbit monoclonal [EPR11755] to RPS16

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC/IF, IP, IHC-P, WBmore details
    Unsuitable for: Flow Cyt

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human RPS16 aa 100 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
    Database link: P62249

  • Positive control

    • MCF-7, fetal liver, HeLa, Ramos, PC-12 and NIH/3T3 cell lysates; Breast carcinoma tissue; MCF-7 cells.

  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.5% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR11755

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab177951 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100 - 1/250.
IP 1/10 - 1/100.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/1000 - 1/5000. Predicted molecular weight: 16 kDa.
  • Application notes
    Is unsuitable for Flow Cyt.

    • Target

    • Relevance

      Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. RPS16 is a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S9P family of ribosomal proteins. It is located in the cytoplasm. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.

    • Database links

      see all

    • Alternative names

      • 40S ribosomal protein S16 antibody
      • Ribosomal protein S16 antibody
      • RPS 16 antibody
      • S16 antibody

    Images

    • Immunoprecipitation - Anti-RPS16 antibody [EPR11755] (ab177951)
      Immunoprecipitation - Anti-RPS16 antibody [EPR11755] (ab177951)

      Western blot analysis on immunoprecipitation pellet from fetal liver lysate using ab177951.

    • Immunocytochemistry/ Immunofluorescence - Anti-RPS16 antibody [EPR11755] (ab177951)
      Immunocytochemistry/ Immunofluorescence - Anti-RPS16 antibody [EPR11755] (ab177951)

      Immunofluorescent analysis of MCF-7 cells labeling RPS16 with ab177951 at 1/100 dilution.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS16 antibody [EPR11755] (ab177951)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS16 antibody [EPR11755] (ab177951)

      Immunohistochemical analysis of paraffin-embedded breast carcinoma tissue labeling RPS16 with ab177951 at 1/50 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Western blot - Anti-RPS16 antibody [EPR11755] (ab177951)
      Western blot - Anti-RPS16 antibody [EPR11755] (ab177951)
      All lanes : Anti-RPS16 antibody [EPR11755] (ab177951) at 1/1000 dilution

      Lane 1 : MCF-7 cell lysate
      Lane 2 : Fetal liver lysate
      Lane 3 : HeLa cell lysate
      Lane 4 : Ramos cell lysate
      Lane 5 : PC-12 cell lysate
      Lane 6 : NIH/3T3 cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 16 kDa

    References

    ab177951 has not yet been referenced specifically in any publications.

    Publishing research using ab177951? Please let us know so that we can cite the reference in this datasheet.

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