Overview

  • Product name

  • Description

    Rabbit polyclonal to RPS18
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ELISA, IHC-P, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide selected from the center region of Human RPS18, conjugated to KLH (NP_072045.1; P62269).

  • Positive control

    • Mouse spleen tissue lysate This antibody gave a positive result in IHC in the following FFPE tissue: Human normal liver.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab91293 is purified through a Protein A column, followed by peptide affinity purification.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab91293 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/1000.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/100 - 1/500. Predicted molecular weight: 18 kDa.

Target

Images

  • IHC image of RPS18 staining in Human normal liver formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab91293, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Anti-RPS18 antibody (ab91293) at 1/100 dilution + Mouse spleen tissue lysate at 35 µg

    Predicted band size: 18 kDa

References

This product has been referenced in:

  • Sinturel F  et al. Diurnal Oscillations in Liver Mass and Cell Size Accompany Ribosome Assembly Cycles. Cell 169:651-663.e14 (2017). Read more (PubMed: 28475894) »
  • Hu X  et al. Common housekeeping proteins are upregulated in colorectal adenocarcinoma and hepatocellular carcinoma, making the total protein a better "housekeeper". Oncotarget 7:66679-66688 (2016). WB . Read more (PubMed: 27556505) »
See all 3 Publications for this product

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A

Application
Immunoprecipitation
Sample
Human Cell lysate - whole cell (293t)
Total protein in input
18 µg
Specification
293t
Immuno-precipitation step
Protein A/G

Mr. Hadar Sinvani

Verified customer

Submitted Mar 13 2013

Answer

Thank you for your quick response and your continuous interest in testing our products.

DISCOUNT CODE: 41879
Expiration date: 27/03/2013
Value: £227.00
I am very pleased to hear you would like to accept our offer and test ab91293 in IP in rabbit. This code will give you £227.00 off your next order before the expiration date. To redeem this offer, please submit an Abreview for IP in rabbit and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active.
For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.
Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.
Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.
The terms and conditions applicable to this offer can be found here: www.abcam.com/abtrial.

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Answer

Thank you for your enquiry and interest.
This customer seems to be interested in testing ab91293 in a new application (IP) and in a new species (rabbit). Even though the homology of the rabbit RPS18 to human is 100%, it is not been properly tested yet. This means that two unknown factors would be introduced into the experimental system. In order to be able to interpret the results correctly, it would be very important to apply a good positive control sample (mouse or human spleen or GI tract tissue lysates). I hope your customer can understand our policy. Please do let me know if the recommendation can be accepted and followed.
I look forward to hearing from you soon.

Read More

Answer

Thank you for contacting Abcam.

Since you are experiencing trouble with GAPHDand alpha-tubulin antibodies, then I would suggest that you try the mitochondrial antibody marker, ab122278, as tubulin is cytosolic but that does not seem to be working for you. However I do suggest that you also consult the latest literature and to see how others in the field are dealing with this issue.

If there is anything else I can help you with, please let me know.

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