Key features and details
- Goat polyclonal to RPS19
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Our Abpromise guarantee covers the use of ab40833 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 15 kDa (predicted molecular weight: 16 kDa).
1 hour primary incubation is recommended for this product.
|IHC-P||Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionRequired for pre-rRNA processing and maturation of 40S ribosomal subunits.
Tissue specificityHigher level expression is seen in the colon carcinoma tissue than normal colon tissue.
Involvement in diseaseDefects in RPS19 are the cause of Diamond-Blackfan anemia type 1 (DBA1) [MIM:105650]. DBA1 is a form of Diamond-Blackfan anemia, a congenital non-regenerative hypoplastic anemia that usually presents early in infancy. Diamond-Blackfan anemia is characterized by a moderate to severe macrocytic anemia, erythroblastopenia, and an increased risk of malignancy. 30 to 40% of Diamond-Blackfan anemia patients present with short stature and congenital anomalies, the most frequent being craniofacial (Pierre-Robin syndrome and cleft palate), thumb and urogenital anomalies.
Sequence similaritiesBelongs to the ribosomal protein S19e family.
Cellular localizationNucleus. Located more specifically in the nucleoli.
- Information by UniProt
- 40S ribosomal protein S19 antibody
- DBA antibody
- DBA1 antibody
Anti-RPS19 antibody (ab40833) at 0.1 µg/ml + K562 cell lysate in RIPA buffer at 35 µg
Predicted band size: 16 kDa
Observed band size: 15 kDa why is the actual band size different from the predicted?
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of Human Tonsil staining RPS19 with ab40833 at 5 μg/ml.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of Human Liver staining RPS19 with ab40833 at 5 μg/ml.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of Human Cortex staining RPS19 with ab40833 at 5 μg/ml.
Anti-RPS19 antibody (ab40833) at 0.1 µg/ml + lysate prepared from transfected HEK293 cells, transiently expressing RPS19
Predicted band size: 16 kDa
Observed band size: 18 kDa why is the actual band size different from the predicted?
ab40833 has been referenced in 3 publications.
- Bibikova E et al. TNF-mediated inflammation represses GATA1 and activates p38 MAP kinase in RPS19-deficient hematopoietic progenitors. Blood 124:3791-8 (2014). PubMed: 25270909
- Horos R et al. Ribosomal deficiencies in Diamond-Blackfan anemia impair translation of transcripts essential for differentiation of murine and human erythroblasts. Blood 119:262-72 (2012). WB ; Mouse . PubMed: 22058113
- Ponpuak M et al. Delivery of cytosolic components by autophagic adaptor protein p62 endows autophagosomes with unique antimicrobial properties. Immunity 32:329-41 (2010). ICC/IF ; Mouse . PubMed: 20206555