Anti-RPS19 antibody (ab40833)
Key features and details
- Goat polyclonal to RPS19
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-RPS19 antibody
See all RPS19 primary antibodies -
Description
Goat polyclonal to RPS19 -
Host species
Goat -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow, Cat, Dog, Chimpanzee, Rhesus monkey -
Immunogen
Synthetic peptide: with C-
KMVEKDQDGGRK
sequence from the internal region of Human RPS19. -
Positive control
- WB: K562 cell lysate.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab40833 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB | (2) |
Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 15 kDa (predicted molecular weight: 16 kDa).
1 hour primary incubation is recommended for this product. |
IHC-P |
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
Notes |
---|
WB
Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 15 kDa (predicted molecular weight: 16 kDa). 1 hour primary incubation is recommended for this product. |
IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
-
Function
Required for pre-rRNA processing and maturation of 40S ribosomal subunits. -
Tissue specificity
Higher level expression is seen in the colon carcinoma tissue than normal colon tissue. -
Involvement in disease
Defects in RPS19 are the cause of Diamond-Blackfan anemia type 1 (DBA1) [MIM:105650]. DBA1 is a form of Diamond-Blackfan anemia, a congenital non-regenerative hypoplastic anemia that usually presents early in infancy. Diamond-Blackfan anemia is characterized by a moderate to severe macrocytic anemia, erythroblastopenia, and an increased risk of malignancy. 30 to 40% of Diamond-Blackfan anemia patients present with short stature and congenital anomalies, the most frequent being craniofacial (Pierre-Robin syndrome and cleft palate), thumb and urogenital anomalies. -
Sequence similarities
Belongs to the ribosomal protein S19e family. -
Cellular localization
Nucleus. Located more specifically in the nucleoli. - Information by UniProt
-
Database links
- Entrez Gene: 6223 Human
- Entrez Gene: 100503302 Mouse
- Entrez Gene: 20085 Mouse
- Entrez Gene: 29287 Rat
- Omim: 603474 Human
- SwissProt: Q32PD5 Cow
- SwissProt: P39019 Human
- SwissProt: Q9CZX8 Mouse
see all -
Alternative names
- 40S ribosomal protein S19 antibody
- DBA antibody
- DBA1 antibody
see all
Images
-
Anti-RPS19 antibody (ab40833) at 0.1 µg/ml + K562 cell lysate in RIPA buffer at 35 µg
Predicted band size: 16 kDa
Observed band size: 15 kDa why is the actual band size different from the predicted?
Protocols
Datasheets and documents
-
Datasheet download
References (4)
ab40833 has been referenced in 4 publications.
- Bibikova E et al. TNF-mediated inflammation represses GATA1 and activates p38 MAP kinase in RPS19-deficient hematopoietic progenitors. Blood 124:3791-8 (2014). PubMed: 25270909
- Blalock WL et al. Identification of the PKR nuclear interactome reveals roles in ribosome biogenesis, mRNA processing and cell division. J Cell Physiol 229:1047-60 (2014). PubMed: 24347309
- Horos R et al. Ribosomal deficiencies in Diamond-Blackfan anemia impair translation of transcripts essential for differentiation of murine and human erythroblasts. Blood 119:262-72 (2012). WB ; Mouse . PubMed: 22058113
- Ponpuak M et al. Delivery of cytosolic components by autophagic adaptor protein p62 endows autophagosomes with unique antimicrobial properties. Immunity 32:329-41 (2010). ICC/IF ; Mouse . PubMed: 20206555