Product nameAnti-RPS19 antibody [EPR10422]
See all RPS19 primary antibodies
DescriptionRabbit monoclonal [EPR10422] to RPS19
Tested applicationsSuitable for: Flow Cyt, WBmore details
Unsuitable for: ICC,IHC-P or IP
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human RPS19 aa 1-100. The exact sequence is proprietary.
Database link: P39019
- Recombinant Human RPS19 protein (ab151818) can be used as a positive control in WB. 293T, Caco-2 and K562 cell lysates; permeabilized 293 cells.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at -20ºC.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.5% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab155994 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|WB||1/1000 - 1/10000. Predicted molecular weight: 16 kDa.|
FunctionRequired for pre-rRNA processing and maturation of 40S ribosomal subunits.
Tissue specificityHigher level expression is seen in the colon carcinoma tissue than normal colon tissue.
Involvement in diseaseDefects in RPS19 are the cause of Diamond-Blackfan anemia type 1 (DBA1) [MIM:105650]. DBA1 is a form of Diamond-Blackfan anemia, a congenital non-regenerative hypoplastic anemia that usually presents early in infancy. Diamond-Blackfan anemia is characterized by a moderate to severe macrocytic anemia, erythroblastopenia, and an increased risk of malignancy. 30 to 40% of Diamond-Blackfan anemia patients present with short stature and congenital anomalies, the most frequent being craniofacial (Pierre-Robin syndrome and cleft palate), thumb and urogenital anomalies.
Sequence similaritiesBelongs to the ribosomal protein S19e family.
Cellular localizationNucleus. Located more specifically in the nucleoli.
- Information by UniProt
- 40S ribosomal protein S19 antibody
- DBA antibody
- DBA1 antibody
All lanes : Anti-RPS19 antibody [EPR10422] (ab155994) at 1/1000 dilution
Lane 1 : 293T cell lysate
Lane 2 : Caco-2 cell lysate
Lane 3 : K562 cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 16 kDa
Flow Cytometric analysis of permeabilized 293 cells labeling RPS19 with ab155994 at 1/10 dilution (red) compared with a rabbit IgG negative control (green).
ab155994 has not yet been referenced specifically in any publications.