Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR10422] to RPS19
- Suitable for: Flow Cyt, WB
- Reacts with: Mouse, Rat, Human
Product nameAnti-RPS19 antibody [EPR10422]
See all RPS19 primary antibodies
DescriptionRabbit monoclonal [EPR10422] to RPS19
Tested applicationsSuitable for: Flow Cyt, WBmore details
Unsuitable for: ICC,IHC-P or IP
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human RPS19 aa 1-100. The exact sequence is proprietary.
Database link: P39019
- Recombinant Human RPS19 protein (ab151818) can be used as a positive control in WB. 293T, Caco-2 and K562 cell lysates; permeabilized 293 cells.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab155994 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|WB||1/1000 - 1/10000. Predicted molecular weight: 16 kDa.|
FunctionRequired for pre-rRNA processing and maturation of 40S ribosomal subunits.
Tissue specificityHigher level expression is seen in the colon carcinoma tissue than normal colon tissue.
Involvement in diseaseDefects in RPS19 are the cause of Diamond-Blackfan anemia type 1 (DBA1) [MIM:105650]. DBA1 is a form of Diamond-Blackfan anemia, a congenital non-regenerative hypoplastic anemia that usually presents early in infancy. Diamond-Blackfan anemia is characterized by a moderate to severe macrocytic anemia, erythroblastopenia, and an increased risk of malignancy. 30 to 40% of Diamond-Blackfan anemia patients present with short stature and congenital anomalies, the most frequent being craniofacial (Pierre-Robin syndrome and cleft palate), thumb and urogenital anomalies.
Sequence similaritiesBelongs to the ribosomal protein S19e family.
Cellular localizationNucleus. Located more specifically in the nucleoli.
- Information by UniProt
- 40S ribosomal protein S19 antibody
- DBA antibody
- DBA1 antibody
All lanes : Anti-RPS19 antibody [EPR10422] (ab155994) at 1/1000 dilution
Lane 1 : 293T cell lysate
Lane 2 : Caco-2 cell lysate
Lane 3 : K562 cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 16 kDa
Flow Cytometric analysis of permeabilized 293 cells labeling RPS19 with ab155994 at 1/10 dilution (red) compared with a rabbit IgG negative control (green).
ab155994 has not yet been referenced specifically in any publications.