Overview

  • Product name
    Anti-RPS19 antibody [EPR10422]
    See all RPS19 primary antibodies
  • Description
    Rabbit monoclonal [EPR10422] to RPS19
  • Host species
    Rabbit
  • Tested applications
    Suitable for: Flow Cyt, WBmore details
    Unsuitable for: ICC,IHC-P or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human RPS19 aa 1-100. The exact sequence is proprietary.
    Database link: P39019

  • Positive control
    • Recombinant Human RPS19 protein (ab151818) can be used as a positive control in WB. 293T, Caco-2 and K562 cell lysates; permeabilized 293 cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at -20ºC.
  • Storage buffer
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.5% BSA
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Monoclonal
  • Clone number
    EPR10422
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab155994 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

WB 1/1000 - 1/10000. Predicted molecular weight: 16 kDa.
  • Application notes
    Is unsuitable for ICC,IHC-P or IP.
  • Target

    • Function
      Required for pre-rRNA processing and maturation of 40S ribosomal subunits.
    • Tissue specificity
      Higher level expression is seen in the colon carcinoma tissue than normal colon tissue.
    • Involvement in disease
      Defects in RPS19 are the cause of Diamond-Blackfan anemia type 1 (DBA1) [MIM:105650]. DBA1 is a form of Diamond-Blackfan anemia, a congenital non-regenerative hypoplastic anemia that usually presents early in infancy. Diamond-Blackfan anemia is characterized by a moderate to severe macrocytic anemia, erythroblastopenia, and an increased risk of malignancy. 30 to 40% of Diamond-Blackfan anemia patients present with short stature and congenital anomalies, the most frequent being craniofacial (Pierre-Robin syndrome and cleft palate), thumb and urogenital anomalies.
    • Sequence similarities
      Belongs to the ribosomal protein S19e family.
    • Cellular localization
      Nucleus. Located more specifically in the nucleoli.
    • Information by UniProt
    • Database links
    • Alternative names
      • 40S ribosomal protein S19 antibody
      • DBA antibody
      • DBA1 antibody
      • HGNC:10402 antibody
      • Ribosomal protein S19 antibody
      • rps19 antibody
      • RS19_HUMAN antibody
      • S19 antibody
      see all

    Images

    • All lanes : Anti-RPS19 antibody [EPR10422] (ab155994) at 1/1000 dilution

      Lane 1 : 293T cell lysate
      Lane 2 : Caco-2 cell lysate
      Lane 3 : K562 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 16 kDa

    • Flow Cytometric analysis of permeabilized 293 cells labeling RPS19 with ab155994 at 1/10 dilution (red) compared with a rabbit IgG negative control (green).

    References

    ab155994 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab155994.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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