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    rps2-antibody-ab154972.pdf

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Immunology Innate Immunity Macrophage / Inflamm.
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Anti-RPS2 antibody (ab154972)

  • Datasheet
  • SDS
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Western blot - Anti-RPS2 antibody (ab154972)
  • Western blot - Anti-RPS2 antibody (ab154972)
  • Immunocytochemistry/ Immunofluorescence - Anti-RPS2 antibody (ab154972)

Key features and details

  • Rabbit polyclonal to RPS2
  • Suitable for: ICC/IF, WB
  • Reacts with: Mouse, Human
  • Isotype: IgG

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Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

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Overview

  • Product name

    Anti-RPS2 antibody
    See all RPS2 primary antibodies
  • Description

    Rabbit polyclonal to RPS2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Cow, Xenopus laevis, Monkey, Zebrafish, Xenopus tropicalis
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 73-293 of Human RPS2 (UniProt P15880).

  • Positive control

    • 293T, A431, Jurkat, Raji and mouse brain lysates; A431 cells.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Innate Immunity
    • Macrophage / Inflamm.
    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • Translation
    • Ribosome

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • Raji whole cell lysate (ab30124)
    • Mouse brain tissue lysate - total protein (ab30151)
    • Jurkat whole cell lysate (ab7899)
    • A-431 whole cell lysate (ab7909)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab154972 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
1/100 - 1/1000.
WB
1/500 - 1/3000. Predicted molecular weight: 31 kDa.
Notes
ICC/IF
1/100 - 1/1000.
WB
1/500 - 1/3000. Predicted molecular weight: 31 kDa.

Target

  • Sequence similarities

    Belongs to the ribosomal protein S5P family.
    Contains 1 S5 DRBM domain.
  • Target information above from: UniProt accession P15880 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 286867 Cow
    • Entrez Gene: 6187 Human
    • Entrez Gene: 16898 Mouse
    • Entrez Gene: 83789 Rat
    • Entrez Gene: 380576 Xenopus laevis
    • Entrez Gene: 493255 Xenopus tropicalis
    • Omim: 603624 Human
    • SwissProt: O18789 Cow
    • SwissProt: P15880 Human
    • SwissProt: P25444 Mouse
    • SwissProt: P27952 Rat
    • Unigene: 356366 Human
    • Unigene: 381079 Human
    • Unigene: 498569 Human
    • Unigene: 506997 Human
    • Unigene: 721310 Human
    • Unigene: 157452 Mouse
    • Unigene: 328846 Mouse
    • Unigene: 389704 Mouse
    • Unigene: 2115 Rat
    • Unigene: 220062 Rat
    see all
  • Alternative names

    • 40S ribosomal protein S2 antibody
    • 40S ribosomal protein S4 antibody
    • LLREP3 antibody
    • LLRep3 protein antibody
    • MGC102851 antibody
    • MGC117344 antibody
    • MGC117345 antibody
    • OK/KNS-cl.6 antibody
    • Protein LLRep3 antibody
    • Ribosomal protein S2 antibody
    • rps2 antibody
    • RPS4 antibody
    • RS2 antibody
    • RS2_HUMAN antibody
    • S2 antibody
    • S4 antibody
    see all

Images

  • Western blot - Anti-RPS2 antibody (ab154972)
    Western blot - Anti-RPS2 antibody (ab154972)
    All lanes : Anti-RPS2 antibody (ab154972) at 1/1000 dilution

    Lane 1 : 293T whole cell lysate
    Lane 2 : A431 whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 31 kDa



    12% SDS PAGE
  • Western blot - Anti-RPS2 antibody (ab154972)
    Western blot - Anti-RPS2 antibody (ab154972)
    Anti-RPS2 antibody (ab154972) at 1/500 dilution + Mouse brain cell lysate at 50 µg

    Predicted band size: 31 kDa



    12% SDS PAGE
  • Immunocytochemistry/ Immunofluorescence - Anti-RPS2 antibody (ab154972)
    Immunocytochemistry/ Immunofluorescence - Anti-RPS2 antibody (ab154972)
    Immunofluorescent analysis of methanol-fixed A431 cells labeling RPS2 with ab154972 at 1/200 dilution. Lower panel co-stained with Hoechst 33342.

Protocols

  • Western blot protocols
  • Immunocytochemistry & immunofluorescence protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (1)

Publishing research using ab154972? Please let us know so that we can cite the reference in this datasheet.

ab154972 has been referenced in 1 publication.

  • Shcherbakov D  et al. Ribosomal mistranslation leads to silencing of the unfolded protein response and increased mitochondrial biogenesis. Commun Biol 2:381 (2019). PubMed: 31637312

Customer reviews and Q&As

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