Anti-RPS2 antibody (ab154972)
Key features and details
- Rabbit polyclonal to RPS2
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-RPS2 antibody
See all RPS2 primary antibodies -
Description
Rabbit polyclonal to RPS2 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Cow, Xenopus laevis, Monkey, Zebrafish, Xenopus tropicalis
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Immunogen
Recombinant fragment, corresponding to a region within amino acids 73-293 of Human RPS2 (UniProt P15880).
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Positive control
- 293T, A431, Jurkat, Raji and mouse brain lysates; A431 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab154972 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF |
1/100 - 1/1000.
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| WB |
1/500 - 1/3000. Predicted molecular weight: 31 kDa.
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| Notes |
|---|
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ICC/IF
1/100 - 1/1000. |
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WB
1/500 - 1/3000. Predicted molecular weight: 31 kDa. |
Target
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Sequence similarities
Belongs to the ribosomal protein S5P family.
Contains 1 S5 DRBM domain. - Information by UniProt
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Database links
- Entrez Gene: 286867 Cow
- Entrez Gene: 6187 Human
- Entrez Gene: 16898 Mouse
- Entrez Gene: 83789 Rat
- Entrez Gene: 380576 Xenopus laevis
- Entrez Gene: 493255 Xenopus tropicalis
- Omim: 603624 Human
- SwissProt: O18789 Cow
see all -
Alternative names
- 40S ribosomal protein S2 antibody
- 40S ribosomal protein S4 antibody
- LLREP3 antibody
see all
Images
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Western blot - Anti-RPS2 antibody (ab154972)All lanes : Anti-RPS2 antibody (ab154972) at 1/1000 dilution
Lane 1 : 293T whole cell lysate
Lane 2 : A431 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 31 kDa
12% SDS PAGE -
Western blot - Anti-RPS2 antibody (ab154972)Anti-RPS2 antibody (ab154972) at 1/500 dilution + Mouse brain cell lysate at 50 µg
Predicted band size: 31 kDa
12% SDS PAGE -
Immunocytochemistry/ Immunofluorescence - Anti-RPS2 antibody (ab154972)Immunofluorescent analysis of methanol-fixed A431 cells labeling RPS2 with ab154972 at 1/200 dilution. Lower panel co-stained with Hoechst 33342.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab154972 has been referenced in 1 publication.
- Shcherbakov D et al. Ribosomal mistranslation leads to silencing of the unfolded protein response and increased mitochondrial biogenesis. Commun Biol 2:381 (2019). PubMed: 31637312