Recombinant Anti-RPS2 antibody [EPR10834(B)] (ab155961)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR10834(B)] to RPS2
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
Overview
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Product name
Anti-RPS2 antibody [EPR10834(B)]
See all RPS2 primary antibodies -
Description
Rabbit monoclonal [EPR10834(B)] to RPS2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details
Unsuitable for: Flow Cyt or IP -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide corresponding to residues in Human RPS2 (P15880)
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Positive control
- Recombinant Human RPS2 protein (ab116767) can be used as a positive control in WB. HeLa, A549 or 293T cell lysate, Human kidney or pancreas tissue, HeLa cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR10834(B) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab155961 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. Predicted molecular weight: 31 kDa.
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IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Use of an HRP/AP polymerized secondary antibody recommended.
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ICC/IF |
1/100 - 1/250.
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Notes |
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WB
1/1000 - 1/10000. Predicted molecular weight: 31 kDa. |
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Use of an HRP/AP polymerized secondary antibody recommended. |
ICC/IF
1/100 - 1/250. |
Target
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Sequence similarities
Belongs to the ribosomal protein S5P family.
Contains 1 S5 DRBM domain. - Information by UniProt
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Database links
- Entrez Gene: 6187 Human
- Entrez Gene: 16898 Mouse
- Entrez Gene: 83789 Rat
- Omim: 603624 Human
- SwissProt: P15880 Human
- SwissProt: P25444 Mouse
- SwissProt: P27952 Rat
- Unigene: 356366 Human
see all -
Alternative names
- 40S ribosomal protein S2 antibody
- 40S ribosomal protein S4 antibody
- LLREP3 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS2 antibody [EPR10834(B)] (ab155961)
Immunohistochemical analysis of paraffin embedded Human kidney tissue labeling RPS2 with ab155961 at a 1/1000 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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All lanes : Anti-RPS2 antibody [EPR10834(B)] (ab155961) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : A549 cell lysate
Lane 3 : 293T cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 31 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS2 antibody [EPR10834(B)] (ab155961)
Immunohistochemical analysis of paraffin embedded Human pancreas tissue labeling RPS2 with ab155961 at a 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunofluorescence analysis of HeLa cells labeling RPS2 with ab155961 at a 1/100 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab155961 has been referenced in 1 publication.
- Duncan FE et al. Age-associated dysregulation of protein metabolism in the mammalian oocyte. Aging Cell 16:1381-1393 (2017). WB ; Mouse . PubMed: 28994181