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    rps2-antibody-epr10834b-ab155961.pdf

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Immunology Innate Immunity Macrophage / Inflamm.
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RecombinantRabMAb

Recombinant Anti-RPS2 antibody [EPR10834(B)] (ab155961)

  • Datasheet
  • SDS
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS2 antibody [EPR10834(B)] (ab155961)
  • Western blot - Anti-RPS2 antibody [EPR10834(B)] (ab155961)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS2 antibody [EPR10834(B)] (ab155961)
  • Immunocytochemistry/ Immunofluorescence - Anti-RPS2 antibody [EPR10834(B)] (ab155961)
  • Anti-RPS2 antibody [EPR10834(B)] (ab155961)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR10834(B)] to RPS2
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Human

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Overview

  • Product name

    Anti-RPS2 antibody [EPR10834(B)]
    See all RPS2 primary antibodies
  • Description

    Rabbit monoclonal [EPR10834(B)] to RPS2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide corresponding to residues in Human RPS2 (P15880)

  • Positive control

    • Recombinant Human RPS2 protein (ab116767) can be used as a positive control in WB. HeLa, A549 or 293T cell lysate, Human kidney or pancreas tissue, HeLa cells.
  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR10834(B)
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Innate Immunity
    • Macrophage / Inflamm.
    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • Translation
    • Ribosome

Associated products

  • Alternative Versions

    • Anti-RPS2 antibody [EPR10834(B)] - BSA and Azide free (ab249215)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • HeLa whole cell lysate (ab150035)
    • A549 whole cell lysate (ab7910)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab155961 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 31 kDa.
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Use of an HRP/AP polymerized secondary antibody recommended.
ICC/IF
1/100 - 1/250.
Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 31 kDa.
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Use of an HRP/AP polymerized secondary antibody recommended.
ICC/IF
1/100 - 1/250.
  • Application notes
    Is unsuitable for Flow Cyt or IP.
  • Target

    • Sequence similarities

      Belongs to the ribosomal protein S5P family.
      Contains 1 S5 DRBM domain.
    • Target information above from: UniProt accession P15880 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 6187 Human
      • Entrez Gene: 16898 Mouse
      • Entrez Gene: 83789 Rat
      • Omim: 603624 Human
      • SwissProt: P15880 Human
      • SwissProt: P25444 Mouse
      • SwissProt: P27952 Rat
      • Unigene: 356366 Human
      • Unigene: 381079 Human
      • Unigene: 498569 Human
      • Unigene: 506997 Human
      • Unigene: 721310 Human
      • Unigene: 157452 Mouse
      • Unigene: 328846 Mouse
      • Unigene: 389704 Mouse
      • Unigene: 2115 Rat
      • Unigene: 220062 Rat
      see all
    • Alternative names

      • 40S ribosomal protein S2 antibody
      • 40S ribosomal protein S4 antibody
      • LLREP3 antibody
      • LLRep3 protein antibody
      • MGC102851 antibody
      • MGC117344 antibody
      • MGC117345 antibody
      • OK/KNS-cl.6 antibody
      • Protein LLRep3 antibody
      • Ribosomal protein S2 antibody
      • rps2 antibody
      • RPS4 antibody
      • RS2 antibody
      • RS2_HUMAN antibody
      • S2 antibody
      • S4 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS2 antibody [EPR10834(B)] (ab155961)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS2 antibody [EPR10834(B)] (ab155961)

      Immunohistochemical analysis of paraffin embedded Human kidney tissue labeling RPS2 with ab155961 at a 1/1000 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Western blot - Anti-RPS2 antibody [EPR10834(B)] (ab155961)
      Western blot - Anti-RPS2 antibody [EPR10834(B)] (ab155961)
      All lanes : Anti-RPS2 antibody [EPR10834(B)] (ab155961) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : A549 cell lysate
      Lane 3 : 293T cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 31 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS2 antibody [EPR10834(B)] (ab155961)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS2 antibody [EPR10834(B)] (ab155961)

      Immunohistochemical analysis of paraffin embedded Human pancreas tissue labeling RPS2 with ab155961 at a 1/50 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunocytochemistry/ Immunofluorescence - Anti-RPS2 antibody [EPR10834(B)] (ab155961)
      Immunocytochemistry/ Immunofluorescence - Anti-RPS2 antibody [EPR10834(B)] (ab155961)
      Immunofluorescence analysis of HeLa cells labeling RPS2 with ab155961 at a 1/100 dilution.
    • Anti-RPS2 antibody [EPR10834(B)] (ab155961)
      Anti-RPS2 antibody [EPR10834(B)] (ab155961)

    Protocols

    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (1)

    Publishing research using ab155961? Please let us know so that we can cite the reference in this datasheet.

    ab155961 has been referenced in 1 publication.

    • Duncan FE  et al. Age-associated dysregulation of protein metabolism in the mammalian oocyte. Aging Cell 16:1381-1393 (2017). WB ; Mouse . PubMed: 28994181

    Customer reviews and Q&As

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