RecombinantRabMAb

Recombinant Anti-RPS2 antibody [EPR10834(B)] (ab155961)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS2 antibody [EPR10834(B)] (ab155961)
  • Western blot - Anti-RPS2 antibody [EPR10834(B)] (ab155961)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS2 antibody [EPR10834(B)] (ab155961)
  • Immunocytochemistry/ Immunofluorescence - Anti-RPS2 antibody [EPR10834(B)] (ab155961)
  • Anti-RPS2 antibody [EPR10834(B)] (ab155961)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR10834(B)] to RPS2
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Human

Overview

  • Product name

    Anti-RPS2 antibody [EPR10834(B)]
    See all RPS2 primary antibodies

  • Description

    Rabbit monoclonal [EPR10834(B)] to RPS2

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
    Unsuitable for: Flow Cyt or IP

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat

  • Immunogen

    Synthetic peptide corresponding to residues in Human RPS2 (P15880)

  • Positive control

    • Recombinant Human RPS2 protein (ab116767) can be used as a positive control in WB. HeLa, A549 or 293T cell lysate, Human kidney or pancreas tissue, HeLa cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab155961 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 31 kDa.
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Use of an HRP/AP polymerized secondary antibody recommended.
ICC/IF
1/100 - 1/250.
Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 31 kDa.
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Use of an HRP/AP polymerized secondary antibody recommended.
ICC/IF
1/100 - 1/250.
  • Application notes
    Is unsuitable for Flow Cyt or IP.

    • Target

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS2 antibody [EPR10834(B)] (ab155961)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS2 antibody [EPR10834(B)] (ab155961)

      Immunohistochemical analysis of paraffin embedded Human kidney tissue labeling RPS2 with ab155961 at a 1/1000 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Western blot - Anti-RPS2 antibody [EPR10834(B)] (ab155961)
      Western blot - Anti-RPS2 antibody [EPR10834(B)] (ab155961)
      All lanes : Anti-RPS2 antibody [EPR10834(B)] (ab155961) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : A549 cell lysate
      Lane 3 : 293T cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 31 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS2 antibody [EPR10834(B)] (ab155961)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS2 antibody [EPR10834(B)] (ab155961)

      Immunohistochemical analysis of paraffin embedded Human pancreas tissue labeling RPS2 with ab155961 at a 1/50 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunocytochemistry/ Immunofluorescence - Anti-RPS2 antibody [EPR10834(B)] (ab155961)
      Immunocytochemistry/ Immunofluorescence - Anti-RPS2 antibody [EPR10834(B)] (ab155961)
      Immunofluorescence analysis of HeLa cells labeling RPS2 with ab155961 at a 1/100 dilution.
    • Anti-RPS2 antibody [EPR10834(B)] (ab155961)
      Anti-RPS2 antibody [EPR10834(B)] (ab155961)

    References (1)

    Publishing research using ab155961? Please let us know so that we can cite the reference in this datasheet.

    ab155961 has been referenced in 1 publication.

    • Duncan FE  et al. Age-associated dysregulation of protein metabolism in the mammalian oocyte. Aging Cell 16:1381-1393 (2017). WB ; Mouse . PubMed: 28994181

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