Overview

  • Product name

    Anti-RPS20 antibody [EPR8716]
    See all RPS20 primary antibodies
  • Description

    Rabbit monoclonal [EPR8716] to RPS20
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC, Flow Cytmore details
    Unsuitable for: IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    A synthetic peptide corresponding to residues in Human RPS20 (UniProt ID: P60866)

  • Positive control

    • HeLa, A431, HUVEC and Human placenta lysates. Human cervical carcinoma tissue.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Dissociation constant (KD)

    KD = 2.51 x 10 -10 M
    Learn more about KD
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR8716
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab133776 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 13 kDa.
IHC-P 1/100 - 1/250.
ICC 1/100 - 1/250.
Flow Cyt Use at an assay dependent concentration.
  • Application notes
    Is unsuitable for IP.
  • Target

    Images

    • All lanes : Anti-RPS20 antibody [EPR8716] (ab133776) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : A431 cell lysate
      Lane 3 : HUVEC cell lysate
      Lane 4 : Human placenta cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP-labelled Goat anti-Rabbit at 1/2000 dilution

      Predicted band size: 13 kDa

    • Flow Cytometry analysis of A431 cells (human epidermoid carcinoma) labeling RPS20 with purified ab9133776 at 1/250 dilution(10ug/ml) (red). Cells were fixed with 4% paraformaldehyde  and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488)(ab150077)(1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black)(ab172730) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.

    • Immunohistochemical analysis of paraffin-embedded human cervical carcinoma tissue labelling RPS20 using ab133776 at 1/100 dilution
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    This product has been referenced in:

    • Kido T  et al. The X-linked tumor suppressor TSPX downregulates cancer-drivers/oncogenes in prostate cancer in a C-terminal acidic domain dependent manner. Oncotarget 10:1491-1506 (2019). Read more (PubMed: 30863497) »
    • Juszkiewicz S & Hegde RS Initiation of Quality Control during Poly(A) Translation Requires Site-Specific Ribosome Ubiquitination. Mol Cell 65:743-750.e4 (2017). Read more (PubMed: 28065601) »
    See all 4 Publications for this product

    Customer reviews and Q&As

    1-2 of 2 Abreviews or Q&A

    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (PBMCs)
    Gel Running Conditions
    Reduced Denaturing (4-20%)
    Loading amount
    15 µg
    Specification
    PBMCs
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C

    Abcam user community

    Verified customer

    Submitted Aug 31 2019

    Application
    Western blot
    Sample
    Jaculus jaculus Tissue lysate - whole (muscle)
    Gel Running Conditions
    Reduced Denaturing (4-20%)
    Loading amount
    15 µg
    Specification
    muscle
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 24°C

    Mai Tran

    Verified customer

    Submitted May 08 2017

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Sign up