Anti-RPS23 antibody [CPTC-YWHAE-1] (ab268130)
Key features and details
- Mouse monoclonal [CPTC-YWHAE-1] to RPS23
- Suitable for: WB, IHC-P, Protein Array
- Reacts with: Human
- Isotype: IgG2a
Overview
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Product name
Anti-RPS23 antibody [CPTC-YWHAE-1]
See all RPS23 primary antibodies -
Description
Mouse monoclonal [CPTC-YWHAE-1] to RPS23 -
Host species
Mouse -
Tested applications
Suitable for: WB, IHC-P, Protein Arraymore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein within Rat RPS23 aa 1-143. The exact sequence is proprietary.
Database link: P62268 -
Positive control
- WB: HEK293, PC-3 and A431 cell lysates IHC-P: human testis tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.05% BSA -
Concentration information loading...
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Purity
Protein A/G purified -
Purification notes
Purified from Bioreactor concentrate -
Clonality
Monoclonal -
Clone number
CPTC-YWHAE-1 -
Isotype
IgG2a -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab268130 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 16 kDa.
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IHC-P |
Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Protein Array |
Use at an assay dependent concentration.
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Notes |
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WB
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 16 kDa. |
IHC-P
Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Protein Array
Use at an assay dependent concentration. |
Target
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Sequence similarities
Belongs to the ribosomal protein S12P family. - Information by UniProt
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Database links
- Entrez Gene: 6228 Human
- Omim: 603683 Human
- SwissProt: P62266 Human
- Unigene: 527193 Human
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Alternative names
- 40S ribosomal protein S23 antibody
- FLJ35016 antibody
- Homolog of yeast ribosomal protein S28 antibody
see all
Images
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Formalin-fixed, paraffin-embedded human testis tissue stained for RPS23 using ab268130 at 2 μg/ml in immunohistochemical analysis.
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Protein Array containing more than 19,000 full-length human proteins using ab268130.
Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.
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All lanes : Anti-RPS23 antibody [CPTC-YWHAE-1] (ab268130) at 2 µg/ml
Lane 1 : HEK293 (Human epithelial cell line from embryonic kidney) cell lysate
Lane 2 : PC-3 (Human prostate adenocarcinoma cell line) cell lysate
Lane 3 : A431 (Human epidermoid carcinoma cell line) cell lysate
Predicted band size: 16 kDa
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (1)
ab268130 has been referenced in 1 publication.
- Qi J et al. Integrated analysis of miRNA-mRNA regulatory network and functional verification of miR-338-3p in coronary heart disease. Funct Integr Genomics 23:16 (2022). PubMed: 36562844