Overview

  • Product name

    Anti-RPS24 antibody [EPR16017(B)]
    See all RPS24 primary antibodies
  • Description

    Rabbit monoclonal [EPR16017(B)] to RPS24
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human RPS24 aa 100 to the C-terminus. The exact sequence is proprietary.
    Database link: P62847

  • Positive control

    • Jurkat and 293T whole cell lysates; Mouse colon, rat colon and mouse liver lysates; Human squamous cell carcinoma of cervix, Human tonsil and mouse liver tissues; SH-SY5Y cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR16017(B)
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab196652 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 15 kDa (predicted molecular weight: 15 kDa).
IHC-P 1/50. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF 1/250.

Target

  • Relevance

    Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S24E family of ribosomal proteins. It is located in the cytoplasm. Multiple transcript variants encoding different isoforms have been found for this gene. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. Mutations in this gene result in Diamond-Blackfan anemia.
  • Cellular localization

    Cytosolic small ribosomal subunit
  • Database links

  • Alternative names

    • 40S ribosomal protein S24 antibody
    • DBA3 antibody
    • DKFZp686N1586 antibody
    • ribosomal protein S24 antibody
    • S24 antibody
    see all

Images

  • All lanes : Anti-RPS24 antibody [EPR16017(B)] (ab196652) at 1/5000 dilution

    Lane 1 : Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysate
    Lane 2 : 293T (Human epithelial cells from embryonic kidney) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 15 kDa
    Observed band size: 15 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

  • All lanes : Anti-RPS24 antibody [EPR16017(B)] (ab196652) at 1/1000 dilution

    Lane 1 : Mouse colon lysate
    Lane 2 : Rat colon lysate
    Lane 3 : Mouse liver lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 15 kDa
    Observed band size: 15 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunohistochemical analysis of paraffin-embedded Human squamous cell carcinoma of cervix tissue labeling RPS24 with ab196652 at 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Cytoplasm staining on Human squamous cell carcinoma of cervix tissue is observed. Counter stained with Hematoxylin.

  • Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling RPS24 with ab196652 at 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Cytoplasm staining on Human tonsil tissue is observed. Counter stained with Hematoxylin.

  • Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling RPS24 with ab196652 at 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Cytoplasm staining on mouse liver tissue is observed. Counter stained with Hematoxylin.

  • Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized SH-SY5Y (Human neuroblastoma from bone marrow cells) cells labeling RPS24 with ab196652 at 1/250 dilution, followed by AlexaFluor®488 Goat anti-Rabbit (ab150077) secondary antibody at 1/400 dilution (green). Cytoplasm staining on SH-SY5Y cell line is observed. The nuclear counter stain is DAPI (blue).

References

This product has been referenced in:

See all 3 Publications for this product

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