Key features and details
- Rabbit polyclonal to RPS25
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-RPS25 antibody
See all RPS25 primary antibodies
DescriptionRabbit polyclonal to RPS25
Tested applicationsSuitable for: WB, ICC/IF, IHC-Pmore details
Species reactivityReacts with: Mouse, Rat, Human
- IHC-P: Human lymph node, fallopian tube, cervix and heart tissue. ICC/IF: A-431 cells. WB: HEL, NIH-3T3 and NBT-II cells
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab254671 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.04 - 0.4 µg/ml.|
|ICC/IF||Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100.
|IHC-P||1/1000 - 1/2500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
RelevanceBelongs to the ribosomal protein S25e family.
Cellular localizationcytosolic small ribosomal subunit
- 40S ribosomal protein S25 antibody
Paraffin-embedded human cervix tissue stained for RPS25 using ab254671 at 1/1000 dilution in immunohistochemical analysis.
PFA fixed, Triton X-100 permeabilized A-431 (Human epidermoid carcinoma cell line) cells labeling RPS25 using ab254671 at 4µg/ml (green) in ICC/IF.
Lanes 2-3 : Anti-RPS25 antibody (ab254671) at 0.4 µg/ml
Lane 1 : DNA Ladder
Lane 2 : NIH-3T3 whole cell lysate
Lane 3 : NBT-II whole cell lysate
Paraffin-embedded human fallopian tube tissue stained for RPS25 using ab254671 at 1/1000 dilution in immunohistochemical analysis.
Paraffin-embedded human heart muscle tissue stained for RPS25 using ab254671 at 1/1000 dilution in immunohistochemical analysis.
Paraffin-embedded human lymph node tissue stained for RPS25 using ab254671 at 1/1000 dilution in immunohistochemical analysis.
Lane 2 : Anti-RPS25 antibody (ab254671) at 0.4 µg/ml
Lane 1 : DNA Ladder
Lane 2 : HEL
ab254671 has not yet been referenced specifically in any publications.