Product nameAnti-RPS27/MPS1 antibody
See all RPS27/MPS1 primary antibodies
DescriptionGoat polyclonal to RPS27/MPS1
Tested applicationsSuitable for: ELISA, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
General notesOfficial Gene Symbol - RPS27. LocusLink ID - 6232 (human); 57294 (mouse); 94266 (rat). Gene Ontology terms - structural constituent of ribosome; RNA binding; zinc ion binding; protein biosynthesis; cell proliferation; signal transduction; ribosome; cytosolic small ribosomal subunit (sensu Eukarya); intracellular.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, 5mg/ml Tris, pH 7.3
Concentration information loading...
PurityImmunogen affinity purified
Purification notesPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunizing Peptide (Blocking)
Our Abpromise guarantee covers the use of ab4385 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use at a concentration of 0.05 - 0.2 µg/ml. Detects a band of approximately 12 kDa (predicted molecular weight: 10 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Tissue specificityExpressed in a wide variety of actively proliferating cells and tumor tissues.
Sequence similaritiesBelongs to the ribosomal protein S27e family.
- Information by UniProt
- 2610206D02Rik antibody
- 3200001M24Rik antibody
- 40S ribosomal protein S27 antibody
Anti-RPS27/MPS1 antibody (ab4385) at 0.05 µg/ml + Jurkat lysate (35µg protein in RIPA buffer).
Predicted band size: 10 kDa
Observed band size: 12 kDa why is the actual band size different from the predicted?
Primary incubation was 1 hour. Detected by chemiluminescence.
This product has been referenced in:
- Yang ZY et al. Metallopanstimulin-1 regulates invasion and migration of gastric cancer cells partially through integrin ß4. Carcinogenesis N/A:N/A (2013). Read more (PubMed: 23803695) »
- Shin HS et al. Arginine methylation of ribosomal protein S3 affects ribosome assembly. Biochem Biophys Res Commun 385:273-8 (2009). WB ; Human . Read more (PubMed: 19460357) »