Overview

  • Product name

  • Description

    Goat polyclonal to RPS27/MPS1
  • Host species

    Goat
  • Tested applications

    Suitable for: ELISA, WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide corresponding to Human RPS27/MPS1 aa 2-14 (N terminal).
    Sequence:

    PLAKDLLHPSPEE-C


    (Peptide available as ab23033)

  • General notes

    Official Gene Symbol - RPS27. LocusLink ID - 6232 (human); 57294 (mouse); 94266 (rat). Gene Ontology terms - structural constituent of ribosome; RNA binding; zinc ion binding; protein biosynthesis; cell proliferation; signal transduction; ribosome; cytosolic small ribosomal subunit (sensu Eukarya); intracellular.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, 5mg/ml Tris, pH 7.3
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab4385 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
WB
  • Application notes
    Peptide ELISA: Antibody detection limit dilution 1:64,000.
    WB: Use at a concentration of 0.05 - 0.2 µg/ml. Detects a band of approximately 12 kDa (predicted molecular weight: 10 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    Images

    • Anti-RPS27/MPS1 antibody (ab4385) at 0.05 µg/ml + Jurkat lysate (35µg protein in RIPA buffer).

      Predicted band size: 10 kDa
      Observed band size: 12 kDa
      why is the actual band size different from the predicted?



      Primary incubation was 1 hour. Detected by chemiluminescence.

    References

    This product has been referenced in:

    • Yang ZY  et al. Metallopanstimulin-1 regulates invasion and migration of gastric cancer cells partially through integrin ß4. Carcinogenesis N/A:N/A (2013). Read more (PubMed: 23803695) »
    • Shin HS  et al. Arginine methylation of ribosomal protein S3 affects ribosome assembly. Biochem Biophys Res Commun 385:273-8 (2009). WB ; Human . Read more (PubMed: 19460357) »
    See all 2 Publications for this product

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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