Product nameAnti-RPS28 antibody
See all RPS28 primary antibodies
DescriptionRabbit polyclonal to RPS28
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Cow, Pig, Zebrafish
Synthetic peptide within Human RPS28 aa 19-69. The exact sequence is proprietary.
Database link: P62857
- WB: HeLa, HEK-293T, Jurkat, TCMK-1 and NIH/3T3 cell lysate. IP: HEK-293T cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab241282 was affinity purified using an epitope specific to RPS28 immobilized on solid support.
Our Abpromise guarantee covers the use of ab241282 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/10000 - 1/25000.|
|IP||Use at 2-10 µg/mg of lysate.|
RelevanceRibosomes consist of a small 40S subunit and a large 60S subunit. RPS28 (Ribosomal protein S28) is a component of the 40S subunit. It belongs to the S28E family of ribosomal proteins.
- 40S ribosomal protein S28 antibody
- Ribosomal protein S28 antibody
- RPS 28 antibody
All lanes : Anti-RPS28 antibody (ab241282) at 0.04 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) cell lysate
Lane 4 : TCMK-1 (mouse kidney epithelial cell line) cell lysate
Lane 5 : NIH/3T3 (mouse embryo fibroblast cell line) cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Exposure time: 3 minutes
RPS28 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab241282 at 6 µg/reaction. Western blot was performed from the immunoprecipitate using ab241282 at 0.4 µg/ml.
Lane 1: ab241282 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG IP in HEK-293T whole cell lysate.
Detection: Chemiluminescence with exposure time of 30 seconds.
ab241282 has not yet been referenced specifically in any publications.