Overview

  • Product name

  • Description

    Rabbit polyclonal to RPS28
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Cow, Pig, Drosophila melanogaster, Zebrafish
  • Immunogen

    Synthetic peptide within Human RPS28 aa 19-69. The exact sequence is proprietary.
    Database link: P62857

  • Positive control

    • WB: HeLa, HEK-293T, Jurkat, TCMK-1 and NIH/3T3 whole cell lysate. IP: RPS28/S33 IP in HEK-293T whole cell lysate.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituent: Tris citrate/phosphate

    pH 7-8
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    Antibody was affinity purified using an epitope specific to RPS28 immobilized on solid support.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab241405 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/25000.
IP Use at 2-10 µg/mg of lysate.

Target

Images

  • All lanes : Anti-RPS28 antibody (ab241405) at 0.04 µg/ml

    Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
    Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
    Lane 4 : TCMK-1 (Mouse kidney epithelial cell line) whole cell lysate
    Lane 5 : NIH/3T3 (Mouse embryo fibroblast cell line) whole cell lysate

    Lysates/proteins at 50 µg per lane.

    Developed using the ECL technique.

    Exposure time: 10 seconds


    Prepared using NETN lysis buffer.

  • RPS28 was immunopreciptated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) using ab241405 at 6 µg per reaction. Western blot was performed on the immunoprecipitate using ab241405 at 0.4 µg/mL.

    Lane 1: ab241405 IP in HEK-293T whole cell lysate.
    Lane 2: Control IgG IP in HEK-293T whole cell lysate.

    Detection: Chemiluminescence with an exposure time of 10 seconds.
    Prepared using NETN lysis buffer.

     

     

     

References

ab241405 has not yet been referenced specifically in any publications.

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