Overview

  • Product name

  • Description

    Rabbit polyclonal to RPS3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Pig, Xenopus laevis, Zebrafish, Rhesus monkey, Chlamydomonas reinhardtii, Orangutan, Xenopus tropicalis
  • Immunogen

    Synthetic peptide corresponding to a region within amino acids 1-50 of Human RPS3 (NP_000996.2).

  • Positive control

    • 293T, HeLa and Jurkat whole cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab140676 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 27 kDa.
IP Use at 2-10 µg/mg of lysate.

Target

  • Sequence similarities

    Belongs to the ribosomal protein S3P family.
    Contains 1 KH type-2 domain.
  • Cellular localization

    Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs.
  • Information by UniProt
  • Database links

  • Alternative names

    • 40S ribosomal protein S3 antibody
    • fb13d09 antibody
    • FLJ26283 antibody
    • FLJ27450 antibody
    • IMR 90 ribosomal protein S3 antibody
    • MGC56088 antibody
    • MGC87870 antibody
    • OTTHUMP00000229804 antibody
    • OTTHUMP00000229805 antibody
    • OTTHUMP00000229874 antibody
    • OTTHUMP00000229877 antibody
    • OTTHUMP00000229878 antibody
    • OTTHUMP00000229879 antibody
    • OTTHUMP00000229880 antibody
    • OTTHUMP00000229882 antibody
    • OTTHUMP00000229883 antibody
    • OTTHUMP00000229886 antibody
    • Ribosomal protein S3 antibody
    • rps3 antibody
    • RS3_HUMAN antibody
    • S3 antibody
    • wu:fb13d09 antibody
    • zgc:56088 antibody
    see all

Images

  • All lanes : Anti-RPS3 antibody (ab140676) at 0.1 µg/ml

    Lane 1 : 293T whole cell lysate at 50 µg
    Lane 2 : 293T whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 50 µg
    Lane 4 : Jurkat whole cell lysate at 50 µg

    Developed using the ECL technique.

    Predicted band size: 27 kDa


    Exposure time: 10 seconds
  • Detection of RPS3 in Immunoprecipitates of 293T whole cell lysate (1 mg for IP, 20% of IP loaded) using ab140676 at 6 µg/mg lysate for IP and at 0.4 µg/ml for subsequent Western blot detection.
    Detection: Chemiluminescence with an exposure time of 10 seconds.

References

This product has been referenced in:

  • Kim J & Kim YS Effect of HIV-1 Tat on the formation of the mitotic spindle by interaction with ribosomal protein S3. Sci Rep 8:8680 (2018). IP . Read more (PubMed: 29875444) »
  • Ochkasova AS  et al. The human ribosome can interact with the abasic site in mRNA via a specific peptide of the uS3 protein located near the mRNA entry channel. Biochimie 158:117-125 (2018). Read more (PubMed: 30594661) »
See all 2 Publications for this product

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