Product nameAnti-RPS3 antibody
See all RPS3 primary antibodies
DescriptionRabbit polyclonal to RPS3
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Pig, Xenopus laevis, Zebrafish, Rhesus monkey, Chlamydomonas reinhardtii, Orangutan, Xenopus tropicalis
- 293T, HeLa and Jurkat whole cell lysates.
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab140676 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 27 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
Sequence similaritiesBelongs to the ribosomal protein S3P family.
Contains 1 KH type-2 domain.
Cellular localizationCytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs.
- Information by UniProt
- 40S ribosomal protein S3 antibody
- fb13d09 antibody
- FLJ26283 antibody
All lanes : Anti-RPS3 antibody (ab140676) at 0.1 µg/ml
Lane 1 : 293T whole cell lysate at 50 µg
Lane 2 : 293T whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 50 µg
Lane 4 : Jurkat whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 27 kDa
Exposure time: 10 seconds
Detection of RPS3 in Immunoprecipitates of 293T whole cell lysate (1 mg for IP, 20% of IP loaded) using ab140676 at 6 µg/mg lysate for IP and at 0.4 µg/ml for subsequent Western blot detection.
Detection: Chemiluminescence with an exposure time of 10 seconds.
This product has been referenced in:
- Kim J & Kim YS Effect of HIV-1 Tat on the formation of the mitotic spindle by interaction with ribosomal protein S3. Sci Rep 8:8680 (2018). IP . Read more (PubMed: 29875444) »
- Ochkasova AS et al. The human ribosome can interact with the abasic site in mRNA via a specific peptide of the uS3 protein located near the mRNA entry channel. Biochimie 158:117-125 (2018). Read more (PubMed: 30594661) »