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    rps3-antibody-ab140688.pdf

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Cell Biology Apoptosis Intracellular Associated Proteins
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Anti-RPS3 antibody (ab140688)

  • Datasheet
  • SDS
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Immunoprecipitation - Anti-RPS3 antibody (ab140688)
  • Western blot - Anti-RPS3 antibody (ab140688)

Key features and details

  • Rabbit polyclonal to RPS3
  • Suitable for: WB, IP
  • Reacts with: Human
  • Isotype: IgG

You may also be interested in

Protein
Product image
Recombinant Human RPS3 protein (ab113158)
Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-RPS3 antibody
    See all RPS3 primary antibodies
  • Description

    Rabbit polyclonal to RPS3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Xenopus laevis, Rhesus monkey, Gorilla, Corn, Salamander, Chinese hamster, Orangutan, African bush elephant, Spotted catshark
  • Immunogen

    Synthetic peptide corresponding to a region within amino acids 193-243 of Human RPS3 (NP_000996.2).

  • Positive control

    • 293T, HeLa and Jurkat whole cell lysate (ab7899).
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    pH: 7
    Preservative: 0.09% Sodium azide
    Constituent: 99% Tris citrate/phosphate

    pH 7-8
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Apoptosis
    • Intracellular
    • Associated Proteins
    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • DNA Damage & Repair
    • DNA Damage Response
    • Other
    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • Translation
    • Ribosome

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • HeLa whole cell lysate (ab150035)
    • HeLa whole cell lysate (ab29545)
    • Jurkat whole cell lysate (ab30128)
    • Jurkat whole cell lysate (ab7899)
  • Recombinant Protein

    • Recombinant Human RPS3 protein (ab113158)
  • Related Products

    • Recombinant Human RPS3 protein (ab113158)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab140688 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/2000 - 1/10000. Predicted molecular weight: 27 kDa.
IP
Use at 2-10 µg/mg of lysate.
Notes
WB
1/2000 - 1/10000. Predicted molecular weight: 27 kDa.
IP
Use at 2-10 µg/mg of lysate.

Target

  • Sequence similarities

    Belongs to the ribosomal protein S3P family.
    Contains 1 KH type-2 domain.
  • Cellular localization

    Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs.
  • Target information above from: UniProt accession P23396 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 6188 Human
    • Entrez Gene: 27050 Mouse
    • Entrez Gene: 140654 Rat
    • Omim: 600454 Human
    • SwissProt: P23396 Human
    • SwissProt: P62908 Mouse
    • SwissProt: P62909 Rat
    • Unigene: 546286 Human
    • Unigene: 236868 Mouse
    • Unigene: 127805 Rat
    see all
  • Alternative names

    • 40S ribosomal protein S3 antibody
    • fb13d09 antibody
    • FLJ26283 antibody
    • FLJ27450 antibody
    • IMR 90 ribosomal protein S3 antibody
    • MGC56088 antibody
    • MGC87870 antibody
    • OTTHUMP00000229804 antibody
    • OTTHUMP00000229805 antibody
    • OTTHUMP00000229874 antibody
    • OTTHUMP00000229877 antibody
    • OTTHUMP00000229878 antibody
    • OTTHUMP00000229879 antibody
    • OTTHUMP00000229880 antibody
    • OTTHUMP00000229882 antibody
    • OTTHUMP00000229883 antibody
    • OTTHUMP00000229886 antibody
    • Ribosomal protein S3 antibody
    • rps3 antibody
    • RS3_HUMAN antibody
    • S3 antibody
    • wu:fb13d09 antibody
    • zgc:56088 antibody
    see all

Images

  • Immunoprecipitation - Anti-RPS3 antibody (ab140688)
    Immunoprecipitation - Anti-RPS3 antibody (ab140688)
    Detection of RPS3 in Immunoprecipitates of 293T whole cell lysate (1 mg for IP, 20% of IP loaded) using ab140688 at 6 µg/mg lysate for IP (Lane 1) and at 0.4 µg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP.
    Detection: Chemiluminescence with an exposure time of 10 seconds.
  • Western blot - Anti-RPS3 antibody (ab140688)
    Western blot - Anti-RPS3 antibody (ab140688)
    All lanes : Anti-RPS3 antibody (ab140688) at 0.1 µg/ml

    Lane 1 : 293T whole cell lysate at 50 µg
    Lane 2 : 293T whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 50 µg
    Lane 4 : Jurkat whole cell lysate at 50 µg

    Developed using the ECL technique.

    Predicted band size: 27 kDa


    Exposure time: 10 seconds

Protocols

  • Western blot protocols
  • Immunoprecipitation protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (2)

Publishing research using ab140688? Please let us know so that we can cite the reference in this datasheet.

ab140688 has been referenced in 2 publications.

  • Schult P  et al. microRNA-122 amplifies hepatitis C virus translation by shaping the structure of the internal ribosomal entry site. Nat Commun 9:2613 (2018). PubMed: 29973597
  • Park J  et al. Misfolded polypeptides are selectively recognized and transported toward aggresomes by a CED complex. Nat Commun 8:15730 (2017). PubMed: 28589942

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