Anti-RPS3 antibody (ab140688)
Key features and details
- Rabbit polyclonal to RPS3
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-RPS3 antibody
See all RPS3 primary antibodies -
Description
Rabbit polyclonal to RPS3 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Xenopus laevis, Rhesus monkey, Gorilla, Corn, Salamander, Chinese hamster, Orangutan, African bush elephant, Spotted catshark
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Immunogen
Synthetic peptide corresponding to a region within amino acids 193-243 of Human RPS3 (NP_000996.2).
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Positive control
- 293T, HeLa and Jurkat whole cell lysate (ab7899).
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7-8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab140688 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/2000 - 1/10000. Predicted molecular weight: 27 kDa.
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| IP |
Use at 2-10 µg/mg of lysate.
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| Notes |
|---|
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WB
1/2000 - 1/10000. Predicted molecular weight: 27 kDa. |
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IP
Use at 2-10 µg/mg of lysate. |
Target
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Sequence similarities
Belongs to the ribosomal protein S3P family.
Contains 1 KH type-2 domain. -
Cellular localization
Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. - Information by UniProt
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Database links
- Entrez Gene: 6188 Human
- Entrez Gene: 27050 Mouse
- Entrez Gene: 140654 Rat
- Omim: 600454 Human
- SwissProt: P23396 Human
- SwissProt: P62908 Mouse
- SwissProt: P62909 Rat
- Unigene: 546286 Human
see all -
Alternative names
- 40S ribosomal protein S3 antibody
- fb13d09 antibody
- FLJ26283 antibody
see all
Images
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Immunoprecipitation - Anti-RPS3 antibody (ab140688)Detection of RPS3 in Immunoprecipitates of 293T whole cell lysate (1 mg for IP, 20% of IP loaded) using ab140688 at 6 µg/mg lysate for IP (Lane 1) and at 0.4 µg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP.
Detection: Chemiluminescence with an exposure time of 10 seconds. -
Western blot - Anti-RPS3 antibody (ab140688)All lanes : Anti-RPS3 antibody (ab140688) at 0.1 µg/ml
Lane 1 : 293T whole cell lysate at 50 µg
Lane 2 : 293T whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 50 µg
Lane 4 : Jurkat whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 27 kDa
Exposure time: 10 seconds
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab140688 has been referenced in 2 publications.
- Schult P et al. microRNA-122 amplifies hepatitis C virus translation by shaping the structure of the internal ribosomal entry site. Nat Commun 9:2613 (2018). PubMed: 29973597
- Park J et al. Misfolded polypeptides are selectively recognized and transported toward aggresomes by a CED complex. Nat Commun 8:15730 (2017). PubMed: 28589942