• Product name

  • Description

    Rabbit polyclonal to RPS3
  • Host species

  • Tested applications

    Suitable for: ICC/IF, IHC-P, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Cow, Pig, Xenopus laevis, Non human primates, Zebrafish
  • Immunogen

    Synthetic peptide corresponding to RPS3 aa 50-150 (internal sequence) conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab86941)

  • Positive control

    • This antibody gave a positive signal in the following lysates: Mouse Liver Tissue; Mouse Kidney Tissue; Rat Liver Tissue; Rat Lung Tissue; HepG2 Whole Cell



Our Abpromise guarantee covers the use of ab77772 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 24 kDa (predicted molecular weight: 27 kDa).


  • Sequence similarities

    Belongs to the ribosomal protein S3P family.
    Contains 1 KH type-2 domain.
  • Cellular localization

    Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs.
  • Information by UniProt
  • Database links

  • Alternative names

    • 40S ribosomal protein S3 antibody
    • fb13d09 antibody
    • FLJ26283 antibody
    • FLJ27450 antibody
    • IMR 90 ribosomal protein S3 antibody
    • MGC56088 antibody
    • MGC87870 antibody
    • OTTHUMP00000229804 antibody
    • OTTHUMP00000229805 antibody
    • OTTHUMP00000229874 antibody
    • OTTHUMP00000229877 antibody
    • OTTHUMP00000229878 antibody
    • OTTHUMP00000229879 antibody
    • OTTHUMP00000229880 antibody
    • OTTHUMP00000229882 antibody
    • OTTHUMP00000229883 antibody
    • OTTHUMP00000229886 antibody
    • Ribosomal protein S3 antibody
    • rps3 antibody
    • RS3_HUMAN antibody
    • S3 antibody
    • wu:fb13d09 antibody
    • zgc:56088 antibody
    see all


  • All lanes : Anti-RPS3 antibody (ab77772) at 1 µg/ml

    Lane 1 : Liver (Mouse) Tissue Lysate
    Lane 2 : Kidney (Mouse) Tissue Lysate
    Lane 3 : Liver (Rat) Tissue Lysate
    Lane 4 : Lung (Rat) Tissue Lysate
    Lane 5 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 27 kDa
    Observed band size: 24 kDa
    why is the actual band size different from the predicted?

    We hypothesize that the 24 kDa band represents the mature (cleaved) form of RPS3.
  • ICC/IF image of ab77772 stained MCF7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab77772, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) HeLa and Hek293 cells at 5µg/ml, and in 100% methanol fixed (5 min) MCF7 cells at 5µg/ml.
  • IHC image of RPS3 staining in human liver carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab77772, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.


This product has been referenced in:

  • Kim DY  et al. Advanced proteomic analyses yield a deep catalog of ubiquitylation targets in Arabidopsis. Plant Cell 25:1523-40 (2013). Read more (PubMed: 23667124) »
See 1 Publication for this product

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A


Thank you for your answer.

I would recommend to include a positive control in the experiment, where the expression of RPS3 is known to be expressed, such as liver tissue or cells (HepG2). This way there will be no ambiguity on the results.

In regards to the testing discount, I need to ask you some more details. Basically, if you want to test in a new application, we can provide a discount on a second purchaseif you first purchase the antibody, feedback the results. The discount would be worth a free primary antibody of your choice from our catalog. (https://www.abcam.com/collaborationdiscount)

In order to know whether I can provide it to you, please explain me how you would treat the cells prior to the staining with the antibody. Would you lyse the cells, fix them, etc?

Thank you for your collaboration. I look forward hearing back from you! Please let me know also if you have any question.

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Thank you for your inquiry.

We have tested the ab77772 in immunocytochemistry and can therefore guarantee that it will work in this application. As I am not an expert in stem cell biology, I would recommend to check the literature to see whether the RPS3 protein is present in your cells. I would not want to give you a wrong advice. I am not aware of any publications yet, whichwould have used this antibody neither for the moment. I am sorry.

If the RPS3 protein is present, we will guarantee detection with the ab77772 in IF on PFA fixed cells of Mouse, Rat orHuman origin. It well be that the antibody will also detect the unfixed RPS3 protein, as long as it will have access to the protein (i.e. the cells are permeabilized). We have however not tested this antibody yet against the unfixed protein.

Please let me know in more details, what applications you plan to use and I will see whether I can provide you with a testing discount.

I hope this information is helpful. Please do not hesitate to contact us again, should you have any additional questions or concerns.

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Thank you for contacting us. Because we carry over 70,000 products, it isn't feasible for us to keep small sample sizes of our products.

We are happy to reassure our customers that all of our products are covered by our Abpromise, which guarantees that the product will work in the applications and species specified on the datasheet, or we will offer a replacement, credit, or refund within 6 months of purchase.

Sincethe product is to be used in an untested species, I checked if these antibodies will be eligible for testing in Drosophila melanogaster in our testing discount program. We generally expect a cross-reaction of antibodies when the immunogen shows a 85% homology or higher.

Unfortunately, this is not the case for these two antibodies:

ab74711 shows a homology of 80% and ab77772shows an even smaller homology of68% to Drosophila melanogaster. A consistent cross-reaction of the antibodies with Drosophila melanogaster is therefore very un-likely.

I am sorry that I did not have a positive answer for you on this occasion and hope this information was nevertheless helpful.

Please do not hesitate to contact us again with any other questions.

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Thank you for your enquiry.

For ab77772, I am afraid that we have not tested for isoform detection. I did a sequence comparison with RPS3A protein sequence and found that the homology is only 12%. Therefore, it is unlikely that there will be any cross reactivity.

For ab122218, the immunogen sequence is 100% similar to RPL4 3 isoforms, RPL4-001 (ENSP00000311430),RPL4-201 (ENSP00000412939) , RPL4-202 (ENSP00000403183). However, we do not have any data to show this results.

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