Recombinant
RabMAb

Recombinant Anti-RPS3 antibody [EPR7808] - Ribosome Marker (ab128995)

Overview

  • Product name

    Anti-RPS3 antibody [EPR7808] - Ribosome Marker
    See all RPS3 primary antibodies
  • Description

    Rabbit monoclonal [EPR7808] to RPS3 - Ribosome Marker
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WB, IHC-Pmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    A synthetic peptide corresponding to residues in Human RPS3 (P23396)

  • Positive control

    • WB: HepG2, PC-12 and Neuro-2a lysate IHC-P: Human kidney tissue ICC/IF: Neuro-2a cells
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab128995 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100.
WB 1/1000 - 1/10000. Predicted molecular weight: 27 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for Flow Cyt or IP.
  • Target

    • Sequence similarities

      Belongs to the ribosomal protein S3P family.
      Contains 1 KH type-2 domain.
    • Cellular localization

      Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs.
    • Information by UniProt
    • Database links

    • Alternative names

      • 40S ribosomal protein S3 antibody
      • fb13d09 antibody
      • FLJ26283 antibody
      • FLJ27450 antibody
      • IMR 90 ribosomal protein S3 antibody
      • MGC56088 antibody
      • MGC87870 antibody
      • OTTHUMP00000229804 antibody
      • OTTHUMP00000229805 antibody
      • OTTHUMP00000229874 antibody
      • OTTHUMP00000229877 antibody
      • OTTHUMP00000229878 antibody
      • OTTHUMP00000229879 antibody
      • OTTHUMP00000229880 antibody
      • OTTHUMP00000229882 antibody
      • OTTHUMP00000229883 antibody
      • OTTHUMP00000229886 antibody
      • Ribosomal protein S3 antibody
      • rps3 antibody
      • RS3_HUMAN antibody
      • S3 antibody
      • wu:fb13d09 antibody
      • zgc:56088 antibody
      see all

    Images

    • All lanes : Anti-RPS3 antibody [EPR7808] - Ribosome Marker (ab128995) at 1/1000 dilution

      Lane 1 : HepG2 cell lysate
      Lane 2 : PC-12 cell lysate
      Lane 3 : Neuro-2a cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 27 kDa

    • Immunocytochemistry/ Immunofluorescence analysis of Neuro-2a (mouse neuroblastoma neuroblast) labeling RPS3 with ab128995 at 1/100 dilution. ab150077, Goat anti rabbit IgG (Alexa Fluor® 488) at 1/1000 was used as the secondary antibody. Cells were fixed with 100% Methanol. ab195889, Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at 1/00 was used as counterstain. Nuclei were stained blue with DAPI.
       
      Confocal image showing cytoplasmic staining in Neuro-2a cells.
    • ab128995, at a 1/50 dilution, staining RPS3 in Human kidney, using Immunohistochemistry, Formalin/PFA-fixed paraffin-embedded tissue.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    This product has been referenced in:

    • Matthes F  et al. Inhibition of the MID1 protein complex: a novel approach targeting APP protein synthesis. Cell Death Discov 4:4 (2018). Read more (PubMed: 29531801) »
    See 1 Publication for this product

    Customer reviews and Q&As

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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