Product nameAnti-RPS3A antibody
See all RPS3A primary antibodies
DescriptionRabbit polyclonal to RPS3A
Tested applicationsSuitable for: IP, IHC-P, WBmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Cow, Cat
Synthetic peptide within Human RPS3A aa 214-264. The exact sequence is proprietary.
Database link: P61247
- IHC-P: Human ovarian carcinoma tissue. IP: HEK-293T whole cell lysate. WB: HeLa, HEK-293T, Jurkat, TCMK and NIH/3T3 whole cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab264368 in the following tested applications.
|IP||Use at 2-10 µg/mg of lysate.|
|IHC-P||1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|WB||1/2000 - 1/10000.|
FunctionMay play a role during erythropoiesis through regulation of transcription factor DDIT3.
Sequence similaritiesBelongs to the ribosomal protein S3Ae family.
Cellular localizationCytoplasm. Nucleus. Localized in cytoplasmic mRNP granules containing untranslated mRNAs.
- Information by UniProt
- 40S ribosomal protein S3a antibody
- Fte 1 antibody
- Fte-1 antibody
All lanes : Anti-RPS3A antibody (ab264368) at 0.1 µg/ml
Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 4 : TCMK (Mouse kidney epithelial cell line) whole cell lysate
Lane 5 : NIH/3T3 (Mouse embryo fibroblast cell line) whole cell lysate
Lysates/proteins at 50 µg per lane.
Exposure time: 3 seconds
Cells prepared using NETN lysis buffer.
Immunohistochemical analysis of formalin-fixed, paraffin-embedded human ovarian carcinoma tissue labeling RPS3A with ab264368 at 1/5000 dilution. Detection: DAB.
RPS3A was immunoprecipitated from 1mg of HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate with ab264368 at 6 µg per reaction. Western blot was performed from the immunoprecipitate using ab264368 at 1 µg/ml.
Lane 1: ab264368 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG.
Exposure time: 3 secs.
ab264368 has not yet been referenced specifically in any publications.