Overview

  • Product name

  • Description

    Rabbit polyclonal to RPS3A
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Pig, Zebrafish
  • Immunogen

    Recombinant fragment corresponding to a region within amino acids 14-262 of Human RPS3A (NP_000997).

  • Positive control

    • 293T, A431, H1299, HeLaS3, HepG2, Molt-4 and Raji whole cell lysates; HeLa cells.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab96534 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 30 kDa.
ICC/IF 1/100 - 1/200.

Target

Images

  • All lanes : Anti-RPS3A antibody (ab96534) at 1/1000 dilution

    Lane 1 : 293T whole cell lysate
    Lane 2 : A431 whole cell lysate
    Lane 3 : H1299 whole cell lysate
    Lane 4 : HeLa S3 whole cell lysate
    Lane 5 : Hep G2 whole cell lysate
    Lane 6 : Molt4 whole cell lysate
    Lane 7 : Raji whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 30 kDa



    12% SDS-PAGE
  • ab96534, at 1/200 dilution, staining paraformaldehyde-fixed HeLa cells by Immunofluorescence. Lower image is merged with DNA probe.

References

ab96534 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Application
Western blot
Sample
Mouse Tissue lysate - whole (Liver)
Loading amount
20 µg
Specification
Liver
Gel Running Conditions
Reduced Non-Denaturing (Native) (4-20%)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 24°C

Abcam user community

Verified customer

Submitted Nov 30 2012

Question
Answer

Thank you for your enquiry.

We have not tested if other sub-families could also be detected with the product but according to amino acid sequence homology, these are the percentage of cross reactivity.




http://www.ncbi.nlm.nih.gov/protein/EAX04995.1- 100% homology
http://www.ncbi.nlm.nih.gov/protein/EAX04994.1- 98% homology
http://www.ncbi.nlm.nih.gov/protein/EAX04993.1- 83% homology
http://www.ncbi.nlm.nih.gov/protein/EAX04992.1- 100% homology
http://www.ncbi.nlm.nih.gov/protein/EAX04990.1- 100% homology
http://www.ncbi.nlm.nih.gov/protein/EAX04989.1- 100% homology
http://www.ncbi.nlm.nih.gov/protein/EAX04988.1- 100% homology

I hope this information will be helpful.

Read More
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Prostate)
Specification
Prostate
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Novocastra pH 6 retrieval buffer
Permeabilization
No
Blocking step
(agent) for 10 minute(s) · Concentration: 0.3% · Temperature: 22°C

Dr. Aamir Ahmed

Verified customer

Submitted Oct 19 2011

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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