Anti-RPS3A antibody - N-terminal (ab226356)
Key features and details
- Rabbit polyclonal to RPS3A - N-terminal
- Suitable for: IHC-P, WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-RPS3A antibody - N-terminal
See all RPS3A primary antibodies -
Description
Rabbit polyclonal to RPS3A - N-terminal -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, IPmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Cow, Xenopus laevis, Zebrafish, Xenopus tropicalis -
Immunogen
Synthetic peptide within Human RPS3A aa 1-50 (N terminal). The exact sequence is proprietary.
Database link: P61247 -
Positive control
- WB: HeLa, HEK-293T, Jurkat, TCMK-1 and NIH/3T3 whole cell lysates. IP: HEK-293T whole cell lysate. IHC-P: Human ovarian cancer tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab226356 was affinity purified using an epitope specific to RPS3A immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab226356 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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WB |
1/2000 - 1/10000. Predicted molecular weight: 30 kDa.
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IP |
Use at 2-10 µg/mg of lysate.
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Notes |
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IHC-P
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
1/2000 - 1/10000. Predicted molecular weight: 30 kDa. |
IP
Use at 2-10 µg/mg of lysate. |
Target
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Function
May play a role during erythropoiesis through regulation of transcription factor DDIT3. -
Sequence similarities
Belongs to the ribosomal protein S3Ae family. -
Cellular localization
Cytoplasm. Nucleus. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. - Information by UniProt
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Database links
- Entrez Gene: 6189 Cow
- Entrez Gene: 6189 Human
- Entrez Gene: 20091 Mouse
- Entrez Gene: 29288 Rat
- Entrez Gene: 444841 Xenopus laevis
- Entrez Gene: 493437 Xenopus tropicalis
- Entrez Gene: 337240 Zebrafish
- Omim: 180478 Human
see all -
Alternative names
- 40S ribosomal protein S3a antibody
- Fte 1 antibody
- Fte-1 antibody
see all
Images
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All lanes : Anti-RPS3A antibody - N-terminal (ab226356) at 0.1 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 4 : TCMK-1 (mouse kidney epithelial cell line) whole cell lysate
Lane 5 : NIH/3T3 (mouse embryonic fibroblast cell line) whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 30 kDa
Exposure time: 3 secondsCell lysates were prepared using NETN lysis buffer.
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RPS3A was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (prepared using NETN lysis buffer; 20% of IP loaded) with ab226356 at 6 µg per reaction. Western blot was performed from the immunoprecipitate using ab226356 at 0.4 µg/ml.
Lane 1: ab226356 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG IP in HEK-293T whole cell lysate.
Detection: Chemiluminescence with exposure time of 3 seconds.
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Formalin-fixed, paraffin-embedded human ovarian carcinoma tissue stained for RPS3A using ab226356 at 1/1000 dilution in immunohistochemical analysis. Detection: DAB staining.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab226356 has not yet been referenced specifically in any publications.