Overview

  • Product name

  • Description

    Rabbit polyclonal to RPS4X/SCAR
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cat, Macaque monkey
  • Immunogen

    Synthetic peptide corresponding to Human RPS4X/SCAR aa 81-130. NP_000998.1.
    Sequence:

    TYPAGFMDVI SIDKTGENFR LIYDTKGRFA VHRITPEEAK YKLCKVRKIF


    Database link: P62701

  • Positive control

    • Human pancreas tissue; HUVEC cells; HeLa and COLO cell lysates.
  • General notes

     This product was previously labelled as RPS4X

     

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 49% PBS, 0.87% Sodium chloride, 50% Glycerol

    PBS (without Mg2+, Ca2+)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab211427 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB 1/500 - 1/1000. Predicted molecular weight: 30 kDa.
ICC/IF 1/100 - 1/500.

Target

Images

  • All lanes : Anti-RPS4X/SCAR antibody (ab211427) at 1/500 dilution

    Lane 1 : COLO cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : HeLa cell lysate with Synthesized peptide

    Predicted band size: 30 kDa

  •  
    Immunofluorescent analysis of HUVEC cells labeling RPS4X/SCAR with ab211427 at 1/100 dilution. The image on the right is treated with the synthesized peptide.
  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human pancreas tissue labeling RPS4X/SCAR with ab211427 at 10µg/ml.

References

ab211427 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab211427.
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