Overview

  • Product name
    Anti-RPS4Y1 antibody
    See all RPS4Y1 primary antibodies
  • Description
    Rabbit polyclonal to RPS4Y1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ELISA, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthesized peptide derived from C-terminal of human RPS4Y1.

  • Positive control
    • Extracts from COLO cells, HeLa cells and HUVEC cells; HUVEC cells

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Affinity-purified from rabbit antiserum by affinity chromatography using epitope-specific immunogen.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab74709 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 29 kDa.
ELISA 1/20000.
ICC/IF 1/500 - 1/1000.

Target

  • Sequence similarities
    Belongs to the ribosomal protein S4e family.
    Contains 1 S4 RNA-binding domain.
  • Information by UniProt
  • Database links
    • Alternative names
      • 40S ribosomal protein S4 antibody
      • 40S ribosomal protein S4, Y antibody
      • 40S ribosomal protein S4, Y isoform 1 antibody
      • MGC119100 antibody
      • MGC5070 antibody
      • ribosomal protein S4, Y linked 1 antibody
      • ribosomal protein S4, Y linked antibody
      • ribosomal protein S4, Y-linked 1 antibody
      • ribosomal protein S4Y antibody
      • RPS4Y antibody
      • RPS4Y1 antibody
      • RS4Y1_HUMAN antibody
      • S4 antibody
      • Y isoform 1 antibody
      see all

    Images

    • Western blot - Anti-RPS4Y1 antibody (ab74709)
      Western blot - Anti-RPS4Y1 antibody (ab74709)
      All lanes : Anti-RPS4Y1 antibody (ab74709) at 1/500 dilution

      Lane 1 : Extracts from COLO cells with no peptide
      Lane 2 : Extracts from HeLa cells with no peptide
      Lane 3 : Extracts from HUVEC cells with no peptide
      Lane 4 : Extracts from HUVEC cells with peptide at 5 µg

      Lysates/proteins at 5 µg per lane.

      Predicted band size: 29 kDa
      Observed band size: 29 kDa
      Additional bands at: 45 kDa. We are unsure as to the identity of these extra bands.

    • Immunocytochemistry/ Immunofluorescence - Anti-RPS4Y1 antibody (ab74709)
      Immunocytochemistry/ Immunofluorescence - Anti-RPS4Y1 antibody (ab74709)
      Immunofluorescence analysis of HuvEc cells, using ab74709 at a dilution of 1/500. Left-hand panel: untreated sample; right-hand panel: with immunising peptide.

    References

    ab74709 has not yet been referenced specifically in any publications.

    Publishing research using ab74709? Please let us know so that we can cite the reference in this datasheet.

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