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    rps4y1-antibody-ab74709.pdf

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Epigenetics and Nuclear Signaling DNA / RNA Translation Ribosome
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Anti-RPS4Y1 antibody (ab74709)

  • Datasheet
  • SDS
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Western blot - Anti-RPS4Y1 antibody (ab74709)
  • Immunocytochemistry/ Immunofluorescence - Anti-RPS4Y1 antibody (ab74709)

Key features and details

  • Rabbit polyclonal to RPS4Y1
  • Suitable for: WB, ICC/IF
  • Reacts with: Human
  • Isotype: IgG

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Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

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Overview

  • Product name

    Anti-RPS4Y1 antibody
  • Description

    Rabbit polyclonal to RPS4Y1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthesized peptide derived from C-terminal of human RPS4Y1.

  • Positive control

    • Extracts from COLO cells, HeLa cells and HUVEC cells; HUVEC cells
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS

    Without Mg2+ and Ca2+
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    Affinity-purified from rabbit antiserum by affinity chromatography using epitope-specific immunogen.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • Translation
    • Ribosome

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab74709 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/500 - 1/1000. Predicted molecular weight: 29 kDa.
ICC/IF
1/500 - 1/1000.
Notes
WB
1/500 - 1/1000. Predicted molecular weight: 29 kDa.
ICC/IF
1/500 - 1/1000.

Target

  • Sequence similarities

    Belongs to the ribosomal protein S4e family.
    Contains 1 S4 RNA-binding domain.
  • Target information above from: UniProt accession P22090 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 6192 Human
    • Omim: 470000 Human
    • SwissProt: P22090 Human
    • Unigene: 282376 Human
    • Alternative names

      • 40S ribosomal protein S4 antibody
      • 40S ribosomal protein S4, Y antibody
      • 40S ribosomal protein S4, Y isoform 1 antibody
      • MGC119100 antibody
      • MGC5070 antibody
      • ribosomal protein S4, Y linked 1 antibody
      • ribosomal protein S4, Y linked antibody
      • ribosomal protein S4, Y-linked 1 antibody
      • ribosomal protein S4Y antibody
      • RPS4Y antibody
      • RPS4Y1 antibody
      • RS4Y1_HUMAN antibody
      • S4 antibody
      • Y isoform 1 antibody
      see all

    Images

    • Western blot - Anti-RPS4Y1 antibody (ab74709)
      Western blot - Anti-RPS4Y1 antibody (ab74709)
      All lanes : Anti-RPS4Y1 antibody (ab74709) at 1/500 dilution

      Lane 1 : Extracts from COLO cells with no peptide
      Lane 2 : Extracts from HeLa cells with no peptide
      Lane 3 : Extracts from HUVEC cells with no peptide
      Lane 4 : Extracts from HUVEC cells with peptide at 5 µg

      Lysates/proteins at 5 µg per lane.

      Predicted band size: 29 kDa
      Observed band size: 29 kDa
      Additional bands at: 45 kDa. We are unsure as to the identity of these extra bands.

    • Immunocytochemistry/ Immunofluorescence - Anti-RPS4Y1 antibody (ab74709)
      Immunocytochemistry/ Immunofluorescence - Anti-RPS4Y1 antibody (ab74709)
      Immunofluorescence analysis of HuvEc cells, using ab74709 at a dilution of 1/500. Left-hand panel: untreated sample; right-hand panel: with immunising peptide.

    Protocols

    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (0)

    Publishing research using ab74709? Please let us know so that we can cite the reference in this datasheet.

    ab74709 has not yet been referenced specifically in any publications.

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