Anti-RPS4Y1 antibody (ab74709)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-RPS4Y1 antibody -
Description
Rabbit polyclonal to RPS4Y1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ELISA, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthesized peptide derived from C-terminal of human RPS4Y1.
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Positive control
- Extracts from COLO cells, HeLa cells and HUVEC cells; HUVEC cells
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 0.87% Sodium chloride, PBS
Without Mg2+ and Ca2+ -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Affinity-purified from rabbit antiserum by affinity chromatography using epitope-specific immunogen. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab74709 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/1000. Predicted molecular weight: 29 kDa. | |
| ELISA | 1/20000. | |
| ICC/IF | 1/500 - 1/1000. |
Target
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Sequence similarities
Belongs to the ribosomal protein S4e family.
Contains 1 S4 RNA-binding domain. - Information by UniProt
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Database links
- Entrez Gene: 6192 Human
- Omim: 470000 Human
- SwissProt: P22090 Human
- Unigene: 282376 Human
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Alternative names
- 40S ribosomal protein S4 antibody
- 40S ribosomal protein S4, Y antibody
- 40S ribosomal protein S4, Y isoform 1 antibody
see all
Images
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Western blot - Anti-RPS4Y1 antibody (ab74709)All lanes : Anti-RPS4Y1 antibody (ab74709) at 1/500 dilution
Lane 1 : Extracts from COLO cells with no peptide
Lane 2 : Extracts from HeLa cells with no peptide
Lane 3 : Extracts from HUVEC cells with no peptide
Lane 4 : Extracts from HUVEC cells with peptide at 5 µg
Lysates/proteins at 5 µg per lane.
Predicted band size: 29 kDa
Observed band size: 29 kDa
Additional bands at: 45 kDa. We are unsure as to the identity of these extra bands. -
Immunocytochemistry/ Immunofluorescence - Anti-RPS4Y1 antibody (ab74709)Immunofluorescence analysis of HuvEc cells, using ab74709 at a dilution of 1/500. Left-hand panel: untreated sample; right-hand panel: with immunising peptide.
Datasheets and documents
References
ab74709 has not yet been referenced specifically in any publications.
