Anti-RPS4Y1 antibody (ab74709)
Key features and details
- Rabbit polyclonal to RPS4Y1
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-RPS4Y1 antibody -
Description
Rabbit polyclonal to RPS4Y1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthesized peptide derived from C-terminal of human RPS4Y1.
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Positive control
- Extracts from COLO cells, HeLa cells and HUVEC cells; HUVEC cells
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS
Without Mg2+ and Ca2+ -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Affinity-purified from rabbit antiserum by affinity chromatography using epitope-specific immunogen. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab74709 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/500 - 1/1000. Predicted molecular weight: 29 kDa.
|
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| ICC/IF |
1/500 - 1/1000.
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| Notes |
|---|
|
WB
1/500 - 1/1000. Predicted molecular weight: 29 kDa. |
|
ICC/IF
1/500 - 1/1000. |
Target
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Sequence similarities
Belongs to the ribosomal protein S4e family.
Contains 1 S4 RNA-binding domain. - Information by UniProt
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Database links
- Entrez Gene: 6192 Human
- Omim: 470000 Human
- SwissProt: P22090 Human
- Unigene: 282376 Human
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Alternative names
- 40S ribosomal protein S4 antibody
- 40S ribosomal protein S4, Y antibody
- 40S ribosomal protein S4, Y isoform 1 antibody
see all
Images
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Western blot - Anti-RPS4Y1 antibody (ab74709)All lanes : Anti-RPS4Y1 antibody (ab74709) at 1/500 dilution
Lane 1 : Extracts from COLO cells with no peptide
Lane 2 : Extracts from HeLa cells with no peptide
Lane 3 : Extracts from HUVEC cells with no peptide
Lane 4 : Extracts from HUVEC cells with peptide at 5 µg
Lysates/proteins at 5 µg per lane.
Predicted band size: 29 kDa
Observed band size: 29 kDa
Additional bands at: 45 kDa. We are unsure as to the identity of these extra bands. -
Immunocytochemistry/ Immunofluorescence - Anti-RPS4Y1 antibody (ab74709)Immunofluorescence analysis of HuvEc cells, using ab74709 at a dilution of 1/500. Left-hand panel: untreated sample; right-hand panel: with immunising peptide.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab74709 has not yet been referenced specifically in any publications.