Product nameAnti-RPS4Y1 antibody
DescriptionRabbit polyclonal to RPS4Y1
Tested applicationsSuitable for: WB, ELISA, ICC/IFmore details
Species reactivityReacts with: Human
Synthesized peptide derived from C-terminal of human RPS4Y1.
- Extracts from COLO cells, HeLa cells and HUVEC cells; HUVEC cells
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 0.87% Sodium chloride, PBS
Without Mg2+ and Ca2+
Concentration information loading...
PurityImmunogen affinity purified
Purification notesAffinity-purified from rabbit antiserum by affinity chromatography using epitope-specific immunogen.
Our Abpromise guarantee covers the use of ab74709 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Predicted molecular weight: 29 kDa.|
|ICC/IF||1/500 - 1/1000.|
Sequence similaritiesBelongs to the ribosomal protein S4e family.
Contains 1 S4 RNA-binding domain.
- Information by UniProt
- 40S ribosomal protein S4 antibody
- 40S ribosomal protein S4, Y antibody
- 40S ribosomal protein S4, Y isoform 1 antibody
All lanes : Anti-RPS4Y1 antibody (ab74709) at 1/500 dilution
Lane 1 : Extracts from COLO cells with no peptide
Lane 2 : Extracts from HeLa cells with no peptide
Lane 3 : Extracts from HUVEC cells with no peptide
Lane 4 : Extracts from HUVEC cells with peptide at 5 µg
Lysates/proteins at 5 µg per lane.
Predicted band size: 29 kDa
Observed band size: 29 kDa
Additional bands at: 45 kDa. We are unsure as to the identity of these extra bands.
Immunofluorescence analysis of HuvEc cells, using ab74709 at a dilution of 1/500. Left-hand panel: untreated sample; right-hand panel: with immunising peptide.
ab74709 has not yet been referenced specifically in any publications.