Overview

  • Product name

  • Description

    Rabbit polyclonal to RPS6
  • Host species

    Rabbit
  • Specificity

    ab127980 detects endogenous levels of total S6 Ribosomal Protein protein.
  • Tested applications

    Suitable for: WB, ELISA, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide, corresponding to amino acids at the C-terminus of Human RPS6.

  • Positive control

    • Human Pancreas tissue; HeLa cell extracts, treated with TNF-a 20ng/ml; HeLa cells.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 49% PBS, 50% Glycerol, 0.88% Sodium chloride
    Note: PBS without Mg2+ or Ca2+ .
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab127980 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 29 kDa.
ELISA 1/1000.
IHC-P Use a concentration of 2.5 - 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/500.

Target

Images

  • All lanes : Anti-RPS6 antibody (ab127980) at 1/500 dilution

    Lane 1 : HeLa cell extract, treated with TNF-a 20ng/ml for 2 minutes
    Lane 2 : HeLa cell extract, treated with the synthesized peptide.

    Developed using the ECL technique.

    Predicted band size: 29 kDa

  • ab127980 at 1/100 dilution, staining RPS6 in HeLa cells by Immunofluorescence. The image on the right is treated with synthesized peptide.
  • ab127980 at 2.5µg/ml, staining RPS6 in Formalin-Fixed, Paraffin-Embedded Human pancreas tissue by Immunohistochemistry.

References

ab127980 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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