Overview

  • Product name

  • Description

    Rabbit polyclonal to RPS6
  • Host species

    Rabbit
  • Specificity

    Detects endogenous levels of total RPS6 protein.
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide from around aa. 233~237 (

    R-L-S-S-L

    ) of Human RPS6 (NP_001001.2).

  • Positive control

    • MCF7 cells; extracts from HepG2 and 293 cells; Human lung carcinoma tissue.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 49% PBS, 50% Glycerol, 0.88% Sodium chloride
    Note: PBS without Mg2+ and Ca2+.
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab131470 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 32 kDa (predicted molecular weight: 29 kDa).
IHC-P 1/50 - 1/100.
ICC/IF 1/100 - 1/200.

Target

Images

  • All lanes : Anti-RPS6 antibody (ab131470) at 1/500 dilution

    Lane 1 : Extracts from HepG2 cells
    Lane 2 : Extracts from 293 cells

    Predicted band size: 29 kDa

  • Immunoflourescent analysis of methanol-fixed MCF7 cells labelling RPS6 with ab131470 at 1/100 dilution.
  • Immunohistochemical analysis of paraffin-embedded Human lung carcinoma tissue labelling RPS6 with ab131470 at 1/50 dilution. The image on the right is preincubated with blocking peptide.

References

This product has been referenced in:

  • Francis C  et al. Divergence of RNA localization between rat and mouse neurons reveals the potential for rapid brain evolution. BMC Genomics 15:883 (2014). ICC ; Mouse, Rat . Read more (PubMed: 25301173) »
See 1 Publication for this product

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