Key features and details
- Rabbit polyclonal to RPS6
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-RPS6 antibody
See all RPS6 primary antibodies
DescriptionRabbit polyclonal to RPS6
SpecificityDetects endogenous levels of total RPS6 protein.
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Mouse, Rat, Human
- MCF7 cells; extracts from HepG2 and 293 cells; Human lung carcinoma tissue.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride
PBS without Mg2+ and Ca2+.
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab131470 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Detects a band of approximately 32 kDa (predicted molecular weight: 29 kDa).|
|IHC-P||1/50 - 1/100.|
|ICC/IF||1/100 - 1/200.|
FunctionMay play an important role in controlling cell growth and proliferation through the selective translation of particular classes of mRNA.
Sequence similaritiesBelongs to the ribosomal protein S6e family.
modificationsRibosomal protein S6 is the major substrate of protein kinases in eukaryote ribosomes. The phosphorylation is stimulated by growth factors, tumor promoting agents, and mitogens. It is dephosphorylated at growth arrest.
- Information by UniProt
- 40S ribosomal protein S6 antibody
- Air8 antibody
- NP33 antibody
All lanes : Anti-RPS6 antibody (ab131470) at 1/500 dilution
Lane 1 : Extracts from HepG2 cells
Lane 2 : Extracts from 293 cells
Predicted band size: 29 kDa
Immunocytochemistry/ Immunofluorescence analysis of methanol-fixed MCF7 cells labelling RPS6 with ab131470 at 1/100 dilution.
Immunohistochemical analysis of paraffin-embedded Human lung carcinoma tissue labelling RPS6 with ab131470 at 1/50 dilution. The image on the right is preincubated with blocking peptide.
ab131470 has been referenced in 1 publication.
- Francis C et al. Divergence of RNA localization between rat and mouse neurons reveals the potential for rapid brain evolution. BMC Genomics 15:883 (2014). ICC ; Mouse, Rat . PubMed: 25301173