Overview

  • Product name

  • Description

    Rabbit polyclonal to RPS6
  • Host species

    Rabbit
  • Specificity

    ab59261 detects endogenous levels of total RPS6 protein.
  • Tested applications

    Suitable for: ICC/IF, WB, IHC-P, ELISAmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    A synthesized non-phosphopeptide derived from human RPS6 around the phosphorylation site of serine 240 (R-A-SP-T-S).

  • Positive control

    • HeLa cells extracts; human skeletal muscle tissue This antibody gave a positive result when used in the following formaldehyde fixed cell lines: DU145

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride

    Without Mg+2 and Ca+2
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab59261 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 µg/ml.
WB 1/500 - 1/1000. Detects a band of approximately 30 kDa (predicted molecular weight: 30 kDa).
IHC-P Use at an assay dependent concentration.
ELISA 1/5000.

Target

Images

  • All lanes : Anti-RPS6 antibody (ab59261) at 1/500 dilution

    Lane 1 : Extracts from HeLa cells treated with TNF-a (20ng/ml, 2 min)
    Lane 2 : Extracts from HeLa cells treated with TNF-a (20ng/ml, 2 min) with immunising peptide

    Predicted band size: 30 kDa
    Observed band size: 30 kDa

  • ICC/IF image of ab59261 stained DU145 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab59261 at 1µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue using ab59261 at a dilution of 1/50, in the presence (right) or absence (left) of immunising peptide.
  • All lanes : Anti-RPS6 antibody (ab59261) at 1/500 dilution

    Lane 1 : Purified 40S ribosomes
    Lane 2 : Purified 60S ribosomes (negative control)
    Lane 3 : Purified 80S ribosomes

    Lysates/proteins at 0.02 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit IgG (whole molecule) conjugated to peroxidase at 1/5000 dilution

    Predicted band size: 30 kDa
    Observed band size: 30 kDa

    See Abreview

References

This product has been referenced in:

  • Yu X  et al. Glutaminyl cyclase inhibitor contributes to the regulation of HSP70, HSP90, actin, and ribosome on gene and protein levels in vitro. J Cell Biochem N/A:N/A (2018). Read more (PubMed: 30582198) »
  • Zhou K  et al. MicroRNA-145 inhibits the activation of the mTOR signaling pathway to suppress the proliferation and invasion of invasive pituitary adenoma cells by targeting AKT3 in vivo and in vitro. Onco Targets Ther 10:1625-1635 (2017). Read more (PubMed: 28352194) »
See all 2 Publications for this product

Customer reviews and Q&As

Application
Western blot
Sample
Human Purified protein (purified 80S ribosomes and ribosomal subunits (40S)
Loading amount
0.02 µg
Specification
purified 80S ribosomes and ribosomal subunits (40S
Treatment
puromycin
Gel Running Conditions
Reduced Denaturing (12 %)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100µg/mL · Temperature: 25°C

Abcam user community

Verified customer

Submitted Oct 29 2010

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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