Product nameAnti-RPS6 antibody
See all RPS6 primary antibodies
DescriptionRabbit polyclonal to RPS6
Tested applicationsSuitable for: IHC-P, WB, IPmore details
Species reactivityReacts with: Mouse, Human, Caenorhabditis elegans
Predicted to work with: Rat, Sheep, Rabbit, Goat, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Chimpanzee, Reptile, Rhesus monkey, Gorilla, Orangutan
Synthetic peptide corresponding to a region between residues 100 and 150 of human RPS6. ( NP_001001.2).
- Whole cell lysate from HeLa and mouse NIH3T3 cells. IHC-P: FFPE human skin tissue sections.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab70227 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||1/1000 - 1/10000. Detects a band of approximately 30 kDa (predicted molecular weight: 30 kDa).|
|IP||Use at 2-5 µg/mg of lysate.|
FunctionMay play an important role in controlling cell growth and proliferation through the selective translation of particular classes of mRNA.
Sequence similaritiesBelongs to the ribosomal protein S6e family.
modificationsRibosomal protein S6 is the major substrate of protein kinases in eukaryote ribosomes. The phosphorylation is stimulated by growth factors, tumor promoting agents, and mitogens. It is dephosphorylated at growth arrest.
- Information by UniProt
- 40S ribosomal protein S6 antibody
- Air8 antibody
- NP33 antibody
Anti-RPS6 antibody (ab70227) at 1/1000 dilution + Caenorhabditis elegans whole adult tissue lysate at 200 µg
HRP-conjugated goat anti-rabbit IgG polyclonal at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 30 kDa
Observed band size: 28 kDa why is the actual band size different from the predicted?
Additional bands at: 24 kDa (possible non-specific binding), 26 kDa (possible non-specific binding), 40 kDa (possible non-specific binding)
Exposure time: 40 seconds
IHC image of RPS6 staining in human skin formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab70227, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
All lanes : Anti-RPS6 antibody (ab70227) at 0.04 µg/ml
Lane 1 : Whole cell lysate from HeLa cells at 50 µg
Lane 2 : Whole cell lysate from HeLa cells at 15 µg
Lane 3 : Whole cell lysate from HeLa cells at 5 µg
Lane 4 : Whole cell lysate from 293T cells at 50 µg
Lane 5 : Whole cell lysate from NIH3T3 cells at 50 µg
Predicted band size: 30 kDa
Observed band size: 30 kDa
Exposure time: 30 seconds
Detection of Human RPS6 by Immunoprecipitation. Samples: A) Whole cell lysate from HeLa cells (1 mg/IP; 1/4 of IP loaded) using affinity purified rabbit anti-RPS6 antibody ab70227 at 3 µg/mg lysate for IP and at 0.04 µg/ml for subequent WB detection. Second lane represents IgG IP control.
This product has been referenced in:
- Yu Z et al. Autophagy defects and related genetic variations in renal cell carcinoma with eosinophilic cytoplasmic inclusions. Sci Rep 8:9972 (2018). Read more (PubMed: 29967346) »
- Tiku V et al. Small nucleoli are a cellular hallmark of longevity. Nat Commun 8:16083 (2017). Read more (PubMed: 28853436) »