Immunocytochemistry/ Immunofluorescence abreview for Anti-RPS6 antibody - Ribosome Marker

Immunocytochemistry/ Immunofluorescence

Drosophila melanogaster Cell (S2 D.mel embryonic cell line)

Yes - 0.05% Triton X100

S2 D.mel embryonic cell line

Paraformaldehyde

16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS + 0.05% Triton X100

1/100

1/1000

Non-Abcam antibody was used: Goat anti-Rabbit IgG (H+L) Secondary Antibody, Ale
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 555

Nice dot-like signal but tends to aggregate at moderate to high concentrations. Drosophila S2 cells were fixed with 4% PFA in PBS for 15min and then permeabilized with 3 washes in PBT (0.05% Triton X100 in PBS). Primary antibody was directly incubated with cells at 1/100 concentration in PBT overnight at 4C. Cells were washed again with PBT and conjugated secondary antibody was incubated in PBT for 4hrs at RT.