Application
Immunocytochemistry/ Immunofluorescence
Sample
Drosophila melanogaster Cell (S2 D.mel embryonic cell line)
Permeabilization
Yes - 0.05% Triton X100
Specification
S2 D.mel embryonic cell line
Fixative
Paraformaldehyde
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS + 0.05% Triton X100
Dilution
1/100
Secondary antibody
Dilution
1/1000
Name
Non-Abcam antibody was used: Goat anti-Rabbit IgG (H+L) Secondary Antibody, Ale
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 555
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 555
Additional data
Additional Notes
Nice dot-like signal but tends to aggregate at moderate to high concentrations.
Drosophila S2 cells were fixed with 4% PFA in PBS for 15min and then permeabilized with 3 washes in PBT (0.05% Triton X100 in PBS). Primary antibody was directly incubated with cells at 1/100 concentration in PBT overnight at 4C. Cells were washed again with PBT and conjugated secondary antibody was incubated in PBT for 4hrs at RT.
Kelly Burke
Verified customer
Submitted Jul 22 2015