Product nameAnti-RPS6 (phospho S235 + S236) antibody
See all RPS6 primary antibodies
DescriptionRabbit polyclonal to RPS6 (phospho S235 + S236)
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Chicken, Cow, Xenopus laevis
Synthetic peptide within Human RPS6 (phospho S235 + S236). The exact sequence is proprietary.
Database link: P62753
- WB: HeLa whole cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab264135 in the following tested applications.
|WB||1/2500 - 1/10000. Predicted molecular weight: 29 kDa.|
FunctionMay play an important role in controlling cell growth and proliferation through the selective translation of particular classes of mRNA.
Sequence similaritiesBelongs to the ribosomal protein S6e family.
modificationsRibosomal protein S6 is the major substrate of protein kinases in eukaryote ribosomes. The phosphorylation is stimulated by growth factors, tumor promoting agents, and mitogens. It is dephosphorylated at growth arrest.
- Information by UniProt
- 40S ribosomal protein S6 antibody
- Air8 antibody
- NP33 antibody
All lanes : Anti-RPS6 (phospho S235 + S236) antibody (ab264135) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate, grown in serum containing media
Lane 2 : HeLa whole cell lysate, cultured for 24 hours in serum free
media; At 24h, serum was replaced for 1 h with 50nM Rapamycin
Lane 3 : HeLa whole cell lysate, cultured for 24 hours in serum free media; At 24h, serum was replaced for 1 h without Rapamycin
Lane 4 : HeLa whole cell lysate, cultured for 24 hours in serum free media; after 24h serum starvation was continued with 50nM Rapamycin
Lane 5 : HeLa whole cell lysate, cultured for 24 hours in serum free
media, after 24h serum starvation was continued without Rapamycin
Lysates/proteins at 35 µg per lane.
Predicted band size: 29 kDa
Exposure time: 10 seconds
The blot was stripped and re-blotted for total RPS6 using an affinity purified rabbit anti-RPS6 antibody at 0.04 μg/ml.
Detection: Chemiluminescence with exposure times of 10 seconds (upper panel) or 30 seconds (lower panel).
ab264135 has not yet been referenced specifically in any publications.