Anti-RPS6 (phospho S235 + S236) antibody (ab264135)
Overview
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Product name
Anti-RPS6 (phospho S235 + S236) antibody
See all RPS6 primary antibodies -
Description
Rabbit polyclonal to RPS6 (phospho S235 + S236) -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Chicken, Cow, Xenopus laevis
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Immunogen
Synthetic peptide within Human RPS6 (phospho S235 + S236). The exact sequence is proprietary.
Database link: P62753 -
Positive control
- WB: HeLa whole cell lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab264135 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/2500 - 1/10000. Predicted molecular weight: 29 kDa. |
Target
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Function
May play an important role in controlling cell growth and proliferation through the selective translation of particular classes of mRNA. -
Sequence similarities
Belongs to the ribosomal protein S6e family. -
Post-translational
modificationsRibosomal protein S6 is the major substrate of protein kinases in eukaryote ribosomes. The phosphorylation is stimulated by growth factors, tumor promoting agents, and mitogens. It is dephosphorylated at growth arrest. - Information by UniProt
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Database links
- Entrez Gene: 396148 Chicken
- Entrez Gene: 787914 Cow
- Entrez Gene: 6194 Human
- Entrez Gene: 20104 Mouse
- Entrez Gene: 667739 Mouse
- Entrez Gene: 29304 Rat
- Entrez Gene: 380281 Xenopus laevis
- Omim: 180460 Human
see all -
Alternative names
- 40S ribosomal protein S6 antibody
- Air8 antibody
- NP33 antibody
see all
Images
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Western blot - Anti-RPS6 (phospho S235 + S236) antibody (ab264135)All lanes : Anti-RPS6 (phospho S235 + S236) antibody (ab264135) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate, grown in serum containing media
Lane 2 : HeLa whole cell lysate, cultured for 24 hours in serum free
media; At 24h, serum was replaced for 1 h with 50nM Rapamycin
Lane 3 : HeLa whole cell lysate, cultured for 24 hours in serum free media; At 24h, serum was replaced for 1 h without Rapamycin
Lane 4 : HeLa whole cell lysate, cultured for 24 hours in serum free media; after 24h serum starvation was continued with 50nM Rapamycin
Lane 5 : HeLa whole cell lysate, cultured for 24 hours in serum free
media, after 24h serum starvation was continued without Rapamycin
Lysates/proteins at 35 µg per lane.
Predicted band size: 29 kDa
Exposure time: 10 seconds4-20% SDS-PAGE.
The blot was stripped and re-blotted for total RPS6 using an affinity purified rabbit anti-RPS6 antibody at 0.04 μg/ml.
Detection: Chemiluminescence with exposure times of 10 seconds (upper panel) or 30 seconds (lower panel).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
References
ab264135 has not yet been referenced specifically in any publications.
