For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Customized Products & Partnerships
    Customized Products & Partnerships

    Customized products and commercial partnerships to accelerate your diagnostic and therapeutic programs.

    Customized products

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

Explore the power of knock-out cell lines for your research

  1. Link

    rps6-phospho-s235--s236-antibody-ab264135.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Epigenetics and Nuclear Signaling DNA / RNA Translation Ribosome
Share by email

Anti-RPS6 (phospho S235 + S236) antibody (ab264135)

  • Datasheet
Submit a review Submit a question

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Shipping info

Promotion Information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Western blot - Anti-RPS6 (phospho S235 + S236) antibody (ab264135)

    Key features and details

    • Rabbit polyclonal to RPS6 (phospho S235 + S236)
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

    You may also be interested in

    Primary
    Product image
    Anti-AKT1 + AKT2 + AKT3 antibody [EPR16798] (ab179463)
    Secondary
    Product image
    Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    Primary
    Product image
    Anti-mTOR antibody [Y391] (ab32028)

    View more associated products

    Overview

    • Product name

      Anti-RPS6 (phospho S235 + S236) antibody
      See all RPS6 primary antibodies
    • Description

      Rabbit polyclonal to RPS6 (phospho S235 + S236)
    • Host species

      Rabbit
    • Tested applications

      Suitable for: WBmore details
    • Species reactivity

      Reacts with: Human
      Predicted to work with: Mouse, Rat, Chicken, Cow, Xenopus laevis
    • Immunogen

      Synthetic peptide within Human RPS6 (phospho S235 + S236). The exact sequence is proprietary.
      Database link: P62753

    • Positive control

      • WB: HeLa whole cell lysate.
    • General notes

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
    • Storage buffer

      pH: 6.8
      Preservative: 0.09% Sodium azide
      Constituents: Tris buffered saline, 0.1% BSA
    • Concentration information loading...
    • Purity

      Immunogen affinity purified
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Epigenetics and Nuclear Signaling
      • DNA / RNA
      • Translation
      • Ribosome

    Associated products

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Positive Controls

      • HeLa whole cell lysate (ab150035)
      • HeLa whole cell lysate (ab29545)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab264135 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB
    1/2500 - 1/10000. Predicted molecular weight: 29 kDa.
    Notes
    WB
    1/2500 - 1/10000. Predicted molecular weight: 29 kDa.

    Target

    • Function

      May play an important role in controlling cell growth and proliferation through the selective translation of particular classes of mRNA.
    • Sequence similarities

      Belongs to the ribosomal protein S6e family.
    • Post-translational
      modifications

      Ribosomal protein S6 is the major substrate of protein kinases in eukaryote ribosomes. The phosphorylation is stimulated by growth factors, tumor promoting agents, and mitogens. It is dephosphorylated at growth arrest.
    • Target information above from: UniProt accession P62753 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 396148 Chicken
      • Entrez Gene: 787914 Cow
      • Entrez Gene: 6194 Human
      • Entrez Gene: 20104 Mouse
      • Entrez Gene: 667739 Mouse
      • Entrez Gene: 29304 Rat
      • Entrez Gene: 380281 Xenopus laevis
      • Omim: 180460 Human
      • SwissProt: P47838 Chicken
      • SwissProt: Q5E995 Cow
      • SwissProt: P62753 Human
      • SwissProt: P62754 Mouse
      • SwissProt: P62755 Rat
      • SwissProt: P39017 Xenopus laevis
      • Unigene: 408073 Human
      • Unigene: 379007 Mouse
      • Unigene: 425090 Mouse
      • Unigene: 34341 Rat
      • Unigene: 53779 Xenopus laevis
      see all
    • Alternative names

      • 40S ribosomal protein S6 antibody
      • Air8 antibody
      • NP33 antibody
      • Phosphoprotein NP33 antibody
      • Pp30 antibody
      • Ribosomal protein S6 antibody
      • RP S6 antibody
      • rps6 antibody
      • RS6 antibody
      • RS6_HUMAN antibody
      • S6 antibody
      • S6 Ribosomal Protein antibody
      see all

    Images

    • Western blot - Anti-RPS6 (phospho S235 + S236) antibody (ab264135)
      Western blot - Anti-RPS6 (phospho S235 + S236) antibody (ab264135)
      All lanes : Anti-RPS6 (phospho S235 + S236) antibody (ab264135) at 0.04 µg/ml

      Lane 1 : HeLa whole cell lysate, grown in serum containing media
      Lane 2 : HeLa whole cell lysate, cultured for 24 hours in serum free
      media; At 24h, serum was replaced for 1 h with 50nM Rapamycin
      Lane 3 : HeLa whole cell lysate, cultured for 24 hours in serum free media; At 24h, serum was replaced for 1 h without Rapamycin
      Lane 4 : HeLa whole cell lysate, cultured for 24 hours in serum free media; after 24h serum starvation was continued with 50nM Rapamycin
      Lane 5 : HeLa whole cell lysate, cultured for 24 hours in serum free
      media, after 24h serum starvation was continued without Rapamycin

      Lysates/proteins at 35 µg per lane.

      Predicted band size: 29 kDa


      Exposure time: 10 seconds


      4-20% SDS-PAGE.

      The blot was stripped and re-blotted for total RPS6 using an affinity purified rabbit anti-RPS6 antibody at 0.04 μg/ml.
      Detection: Chemiluminescence with exposure times of 10 seconds (upper panel) or 30 seconds (lower panel).

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet download

      Download

    References (0)

    Publishing research using ab264135? Please let us know so that we can cite the reference in this datasheet.

    ab264135 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    There are currently no Customer reviews or Questions for ab264135.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Get resources and offers direct to your inbox Sign up
    A-Z by research area
    • Cancer
    • Cardiovascular
    • Cell biology
    • Developmental biology
    • Epigenetics & Nuclear signaling
    • Immunology
    • Metabolism
    • Microbiology
    • Neuroscience
    • Signal transduction
    • Stem cells
    A-Z by product type
    • Primary antibodies
    • Secondary antibodies
    • Biochemicals
    • Isotype controls
    • Flow cytometry multi-color selector
    • Kits
    • Loading controls
    • Lysates
    • Peptides
    • Proteins
    • Slides
    • Tags and cell markers
    • Tools & Reagents
    Help & support
    • Support
    • Make an Inquiry
    • Protocols & troubleshooting
    • Placing an order
    • RabMAb products
    • Biochemical product FAQs
    • Training
    • Browse by Target
    Company
    • Corporate site
    • Investor relations
    • Company news
    • Careers
    • About us
    • Blog
    Events
    • Tradeshows
    • Conferences
    International websites
    • abcam.cn
    • abcam.co.jp

    Join with us

    • LinkedIn
    • facebook
    • Twitter
    • YouTube
    • Terms of sale
    • Website terms of use
    • Cookie policy
    • Privacy policy
    • Legal
    • Modern slavery statement
    © 1998-2022 Abcam plc. All rights reserved.