Recombinant Anti-RPS6 (phospho S235 + S236) antibody [SP50] (ab101691)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS6 (phospho S235 + S236) antibody [SP50] (ab101691)](https://www.abcam.com/ps/products/101/ab101691/Images/ab101691-7-AntiRPS6-phospho-S235--S236-antibody-SP50-IHC-rat-spleen.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS6 (phospho S235 + S236) antibody [SP50] (ab101691)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [SP50] to RPS6 (phospho S235 + S236)
- Suitable for: Dot blot, IHC-P, ICC/IF, Flow Cyt
- Reacts with: Human
Overview
-
Product name
Anti-RPS6 (phospho S235 + S236) antibody [SP50]
See all RPS6 primary antibodies -
Description
Rabbit monoclonal [SP50] to RPS6 (phospho S235 + S236) -
Host species
Rabbit -
Tested applications
Suitable for: Dot blot, IHC-P, ICC/IF, Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human RPS6 aa 200 to the C-terminus (phospho S235 + S236). The exact sequence is proprietary.
Database link: P62753 -
Positive control
- IHC-P: Human breast carcinoma and tonsil tissue; Mouse Spleen tissue; Rat Spleen Tissue ICC/IF: PC-3 cells. Flow Cyt: PC-3 cells.
-
General notes
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.60
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading... -
Purity
Protein A/G purified -
Purification notes
Purified from TCS by protein A/G. -
Clonality
Monoclonal -
Clone number
SP50 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Isotype control
-
Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab101691 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Dot blot | Use a concentration of 0.123 µg/ml. | |
| IHC-P | 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| ICC/IF | 1/500. | |
| Flow Cyt | 1/200. |
Target
-
Function
May play an important role in controlling cell growth and proliferation through the selective translation of particular classes of mRNA. -
Sequence similarities
Belongs to the ribosomal protein S6e family. -
Post-translational
modificationsRibosomal protein S6 is the major substrate of protein kinases in eukaryote ribosomes. The phosphorylation is stimulated by growth factors, tumor promoting agents, and mitogens. It is dephosphorylated at growth arrest. - Information by UniProt
-
Database links
- Entrez Gene: 6194 Human
- Omim: 180460 Human
- SwissProt: P62753 Human
- Unigene: 408073 Human
-
Alternative names
- 40S ribosomal protein S6 antibody
- Air8 antibody
- NP33 antibody
see all
Images
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS6 (phospho S235 + S236) antibody [SP50] (ab101691)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Rat spleen tissue sections labeling RPS6 with ab101691 at 1/100 dilution (1.23 µg/ml). Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Dako™REAL™ EnVision™ Detection System (K5007) was used as the secondary antibody. Hematoxylin was used as a counterstain. Cytoplasmic staining on human rat spleen without alkaline phosphatase treatment (image A); and no signal was detected when tissues were treated with alkaline phosphatase (image B), performed on a Leica Biosystems BOND™ RX instrument.
The section was incubated with ab101691 for 10 mins at room temperature. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS6 (phospho S235 + S236) antibody [SP50] (ab101691)](https://www.abcam.com/ps/products/101/ab101691/Images/ab101691-6-AntiRPS6-phospho-S235--S236-antibody-SP50-IHC-mouse-stomach.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS6 (phospho S235 + S236) antibody [SP50] (ab101691)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse stomach tissue sections labeling RPS6 with ab101691 at 1/100 dilution (1.23 µg/ml). Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Dako™REAL™ EnVision™ Detection System (K5007) was used as the secondary antibody. Hematoxylin was used as a counterstain. Cytoplasmic staining on mouse stomach without alkaline phosphatase treatment (image A); and no signal was detected when tissues were treated with alkaline phosphatase (image B), performed on a Leica Biosystems BOND™ RX instrument.
The section was incubated with ab101691 for 10 mins at room temperature. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS6 (phospho S235 + S236) antibody [SP50] (ab101691)](https://www.abcam.com/ps/products/101/ab101691/Images/ab101691-5-AntiRPS6-phospho-S235--S236-antibody-SP50-IHC-human-breast-carcinoma.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS6 (phospho S235 + S236) antibody [SP50] (ab101691)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human breast carcinoma tissue sections labeling RPS6 with ab101691 at 1/100 dilution (1.23 µg/ml). Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Dako™ REAL™ EnVision™ Detection System (K5007) was used as the secondary antibody. Hematoxylin was used as a counterstain. Cytoplasmic staining on human breast carcinoma without alkaline phosphatase treatment (image A); and no signal was detected when tissues were treated with alkaline phosphatase (image B), performed on a Leica Biosystems BOND™ RX instrument.
The section was incubated with ab101691 for 10 mins at room temperature. -
![Immunocytochemistry/ Immunofluorescence - Anti-RPS6 (phospho S235 + S236) antibody [SP50] (ab101691)](https://www.abcam.com/ps/products/101/ab101691/Images/ab101691-338477-anti-rps6-phospho-s235-s236-antibody-sp50-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-RPS6 (phospho S235 + S236) antibody [SP50] (ab101691)Immunocytochemistry/ Immunofluorescence analysis of PC-3 (human prostate adenocarcinoma epithelial cell) treated with Lamda phosphatase cells labeling RPS6 with purified ab101691 at 1:500 (0.25 µg/ml). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control. -
![Dot Blot - Anti-RPS6 (phospho S235 + S236) antibody [SP50] (ab101691)](https://www.abcam.com/ps/products/101/ab101691/Images/ab101691-4-antiRPS6phosphoS235S236antibody-dotblot-peptides-human.jpg)
Dot Blot - Anti-RPS6 (phospho S235 + S236) antibody [SP50] (ab101691)Dot blot analysis of RPS6 (phospho S235 + S236) peptide (Lane 1), RPS6 (phospho S235) peptide (phospho 2) RPS6 (phospho S236) peptide (Lane 3) and RPS6 non-phospho peptide (Lane 4) labeling RPS6 (phospho S235 + S236) peptide with purified ab101691 at 0.123 ug/mL. ab97051 was used as the secondary antibody at 0.01 ug/mL.
Blocking buffer: 5% NFDM/TBST. Dilution buffer: 5% NFDM /TBST .
-
![Flow Cytometry - Anti-RPS6 (phospho S235 + S236) antibody [SP50] (ab101691)](https://www.abcam.com/ps/products/101/ab101691/Images/ab101691-338494-anti-rps6-phospho-s235-s236-antibody-sp50-flow-cytometry.jpg)
Flow Cytometry - Anti-RPS6 (phospho S235 + S236) antibody [SP50] (ab101691)Flow Cytometry analysis of PC-3 (Human prostate adenocarcinoma epithelial cell) cells labeling RPS6 with purified ab101691 at 1:200 dilution (0.615 µg/ml) Red. Cells were fixed with 4% paraformaldehyde . A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1:2000 dilution. Isotype control - Rabbit monoclonal IgG (ab172730) / Black. Unlabeled control - Unlabelled cells / Blue. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS6 (phospho S235 + S236) antibody [SP50] (ab101691)](https://www.abcam.com/ps/products/101/ab101691/Images/ab101691-114583-anti-rps6-phospho-s235-s236-antibody-sp50-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS6 (phospho S235 + S236) antibody [SP50] (ab101691)ab101691 at 1/100 dilution staining S6K in Human breast carcinoma by Immunohistochemistry Formalin-fixed, Paraffin-embedded tissue. -
![Anti-RPS6 (phospho S235 + S236) antibody [SP50] (ab101691)](https://www.abcam.com/ps/products/101/ab101691/Images/ab101691-8-benefits-of-recombinant-antibodies.png)
Anti-RPS6 (phospho S235 + S236) antibody [SP50] (ab101691)
References (0)
ab101691 has not yet been referenced specifically in any publications.
