Key features and details
- Rabbit polyclonal to RPS6 (phospho S235)
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-RPS6 (phospho S235) antibody
See all RPS6 primary antibodies
DescriptionRabbit polyclonal to RPS6 (phospho S235)
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Mouse, Rat, Human
- HeLa cells; PC12 cell extract (anisomycin-treated)
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 0.88% Sodium chloride, 50% Glycerol, 49% PBS
PBS without Mg2+ and Ca2+
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab131535 was purified by affinity chromatography using epitope-specific phosphopeptide. Non-phosphospecific antibodies were removed by chromatography using non-phosphopeptide.
Our Abpromise guarantee covers the use of ab131535 in the following tested applications.
|WB||1/500 - 1/1000. Predicted molecular weight: 29 kDa.|
|ICC/IF||1/100 - 1/200.|
FunctionMay play an important role in controlling cell growth and proliferation through the selective translation of particular classes of mRNA.
Sequence similaritiesBelongs to the ribosomal protein S6e family.
modificationsRibosomal protein S6 is the major substrate of protein kinases in eukaryote ribosomes. The phosphorylation is stimulated by growth factors, tumor promoting agents, and mitogens. It is dephosphorylated at growth arrest.
- Information by UniProt
- 40S ribosomal protein S6 antibody
- Air8 antibody
- NP33 antibody
All lanes : Anti-RPS6 (phospho S235) antibody (ab131535) at 1/500 dilution
Lane 1 : PC12 cell extract (untreated)
Lane 2 : PC12 cell extract (anisomycin treated)
Predicted band size: 29 kDa
Immunofluorescence analysis of methanol-fixed HeLa cells labelling RPS6 (phospho S235) with ab131535 at 1/100 dilution.
ab131535 has been referenced in 1 publication.
- Zhou K et al. MicroRNA-145 inhibits the activation of the mTOR signaling pathway to suppress the proliferation and invasion of invasive pituitary adenoma cells by targeting AKT3 in vivo and in vitro. Onco Targets Ther 10:1625-1635 (2017). PubMed: 28352194