Overview

  • Product name

    Anti-RPS6 (phospho S235) antibody
    See all RPS6 primary antibodies
  • Description

    Rabbit polyclonal to RPS6 (phospho S235)
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic phosphopeptide conjugated to KLH, derived from around the phosphorylation site of Serine 235 (

    R-L-S(p)-S-L

    ) of Human RPS6 (NP_001001.2).

  • Positive control

    • HeLa cells; PC12 cell extract (anisomycin-treated)

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 49% PBS, 50% Glycerol, 0.88% Sodium chloride
    Note: PBS without Mg2+ and Ca2+
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab131535 was purified by affinity chromatography using epitope-specific phosphopeptide. Non-phosphospecific antibodies were removed by chromatography using non-phosphopeptide.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab131535 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 29 kDa.
ICC/IF 1/100 - 1/200.

Target

Images

  • All lanes : Anti-RPS6 (phospho S235) antibody (ab131535) at 1/500 dilution

    Lane 1 : PC12 cell extract (untreated)
    Lane 2 : PC12 cell extract (anisomycin treated)

    Predicted band size: 29 kDa

  • Immunofluorescence analysis of methanol-fixed HeLa cells labelling RPS6 (phospho S235) with ab131535 at 1/100 dilution.

References

This product has been referenced in:

  • Zhou K  et al. MicroRNA-145 inhibits the activation of the mTOR signaling pathway to suppress the proliferation and invasion of invasive pituitary adenoma cells by targeting AKT3 in vivo and in vitro. Onco Targets Ther 10:1625-1635 (2017). Read more (PubMed: 28352194) »
See 1 Publication for this product

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