Product nameAnti-RPS6 (phospho S235) antibody - C-terminal
See all RPS6 primary antibodies
DescriptionRabbit polyclonal to RPS6 (phospho S235) - C-terminal
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Chicken, Cow, Dog, Xenopus laevis, Zebrafish
Synthetic peptide within Human RPS6 (C terminal) (phospho S235). The exact sequence is proprietary. Conjugated to a protein carrier.
Database link: P62753
- IHC-P: Mouse brain tissue. WB: HEK-293T, starved for 16 hours, whole cell extract.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.025% Proclin
Constituents: PBS, 1% BSA, 20% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab227005 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 29 kDa.|
|IHC-P||1/100 - 1/1000.|
FunctionMay play an important role in controlling cell growth and proliferation through the selective translation of particular classes of mRNA.
Sequence similaritiesBelongs to the ribosomal protein S6e family.
modificationsRibosomal protein S6 is the major substrate of protein kinases in eukaryote ribosomes. The phosphorylation is stimulated by growth factors, tumor promoting agents, and mitogens. It is dephosphorylated at growth arrest.
- Information by UniProt
- 40S ribosomal protein S6 antibody
- Air8 antibody
- NP33 antibody
Paraffin-embedded mouse brain tissue stained for RPS6 (phospho S235) using ab227005 at 1/500 dilution in immunohistochemical analysis.
All lanes : Anti-RPS6 (phospho S235) antibody - C-terminal (ab227005) at 1/1000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen), starved for 16 hours, whole cell extract
Lane 2 : HEK-293T, starved for 16 hours and treated with 100 nM Rapamycin for 30 minutes, whole cell extract
Lane 3 : HEK-293T, starved for 16 hours and treated with 100 nM Insulin for 30 minutes, whole cell extract
Lane 4 : HEK-293T, starved for 16 hours and treated with 100 nM Rapamycin for 30 minutes and 100 nM Insulin for 30 minutes, whole cell extract
Lysates/proteins at 40 µg per lane.
Predicted band size: 29 kDa
12% SDS-PAGE gel.
ab227005 has not yet been referenced specifically in any publications.