Key features and details
- Rabbit polyclonal to RPS6 (phospho S235) - C-terminal
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-RPS6 (phospho S235) antibody - C-terminal
See all RPS6 primary antibodies
DescriptionRabbit polyclonal to RPS6 (phospho S235) - C-terminal
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Chicken, Cow, Dog, Xenopus laevis, Zebrafish
Synthetic peptide within Human RPS6 (C terminal) (phospho S235). The exact sequence is proprietary. Conjugated to a protein carrier.
Database link: P62753
- IHC-P: Mouse brain tissue. WB: HEK-293T, starved for 16 hours, whole cell extract.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab227005 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 29 kDa.|
|IHC-P||1/100 - 1/1000.|
FunctionMay play an important role in controlling cell growth and proliferation through the selective translation of particular classes of mRNA.
Sequence similaritiesBelongs to the ribosomal protein S6e family.
modificationsRibosomal protein S6 is the major substrate of protein kinases in eukaryote ribosomes. The phosphorylation is stimulated by growth factors, tumor promoting agents, and mitogens. It is dephosphorylated at growth arrest.
- Information by UniProt
- 40S ribosomal protein S6 antibody
- Air8 antibody
- NP33 antibody
Paraffin-embedded mouse brain tissue stained for RPS6 (phospho S235) using ab227005 at 1/500 dilution in immunohistochemical analysis.
All lanes : Anti-RPS6 (phospho S235) antibody - C-terminal (ab227005) at 1/1000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen), starved for 16 hours, whole cell extract
Lane 2 : HEK-293T, starved for 16 hours and treated with 100 nM Rapamycin for 30 minutes, whole cell extract
Lane 3 : HEK-293T, starved for 16 hours and treated with 100 nM Insulin for 30 minutes, whole cell extract
Lane 4 : HEK-293T, starved for 16 hours and treated with 100 nM Rapamycin for 30 minutes and 100 nM Insulin for 30 minutes, whole cell extract
Lysates/proteins at 40 µg per lane.
Predicted band size: 29 kDa
12% SDS-PAGE gel.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab227005 has not yet been referenced specifically in any publications.