Product nameAnti-RPS6 (phospho S235) antibody [EP1338(2)Y] - BSA and Azide free
See all RPS6 primary antibodies
DescriptionRabbit monoclonal [EP1338(2)Y] to RPS6 (phospho S235) - BSA and Azide free
Tested applicationsSuitable for: Dot blot, WB, IP, IHC-Pmore details
Unsuitable for: Flow Cyt
Species reactivityReacts with: Human
Synthetic peptide within Human RPS6. The exact sequence is proprietary.
- Human breast adenocarcinoma tissue; HeLa cell lysate, untreated or treated with Calyculin A. This antibody gave a positive result when used in the following formaldehyde fixed cell lines: DU145
Ab213165 is the carrier-free version of ab80158. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Concentration information loading...
Our Abpromise guarantee covers the use of ab213165 in the following tested applications.
|Dot blot||Use at an assay dependent concentration.|
|WB||Use at an assay dependent concentration. Detects a band of approximately 30 kDa (predicted molecular weight: 30 kDa).|
|IP||Use at an assay dependent concentration.|
|IHC-P||Use at an assay dependent concentration.|
FunctionMay play an important role in controlling cell growth and proliferation through the selective translation of particular classes of mRNA.
Sequence similaritiesBelongs to the ribosomal protein S6e family.
modificationsRibosomal protein S6 is the major substrate of protein kinases in eukaryote ribosomes. The phosphorylation is stimulated by growth factors, tumor promoting agents, and mitogens. It is dephosphorylated at growth arrest.
- Information by UniProt
- 40S ribosomal protein S6 antibody
- Air8 antibody
- NP33 antibody
Dot Blot analysis of -
Lane 1: Rb (pS235) phospho peptide
Lane 2: Rb (pS236) phospho peptide
Lane 3: Rb (pS235 & pS236) phospho peptide
Lane 4: Rb non-phospho peptide
5% NFDM/TBST was used as the diluting and blocking buffer.
ab97051 Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated was used as the secondary antibody at 1/100000 dilution. Exposure time: 3 minutes.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab80158).
ab213165 has not yet been referenced specifically in any publications.