Recombinant
RabMAb

Recombinant Anti-RPS6 (phospho S235) antibody [EP1338(2)Y] - BSA and Azide free (ab213165)

Overview

  • Product name

    Anti-RPS6 (phospho S235) antibody [EP1338(2)Y] - BSA and Azide free
    See all RPS6 primary antibodies
  • Description

    Rabbit monoclonal [EP1338(2)Y] to RPS6 (phospho S235) - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Dot blot, WB, IP, IHC-Pmore details
    Unsuitable for: Flow Cyt
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human RPS6. The exact sequence is proprietary.

  • Positive control

    • Human breast adenocarcinoma tissue; HeLa cell lysate, untreated or treated with Calyculin A. This antibody gave a positive result when used in the following formaldehyde fixed cell lines: DU145
  • General notes

    Ab213165 is the carrier-free version of ab80158. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Affinity purified
  • Clonality

    Monoclonal
  • Clone number

    EP1338(2)Y
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab213165 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Dot blot Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Detects a band of approximately 30 kDa (predicted molecular weight: 30 kDa).
IP Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.
  • Application notes
    Is unsuitable for Flow Cyt.
  • Target

    • Function

      May play an important role in controlling cell growth and proliferation through the selective translation of particular classes of mRNA.
    • Sequence similarities

      Belongs to the ribosomal protein S6e family.
    • Post-translational
      modifications

      Ribosomal protein S6 is the major substrate of protein kinases in eukaryote ribosomes. The phosphorylation is stimulated by growth factors, tumor promoting agents, and mitogens. It is dephosphorylated at growth arrest.
    • Information by UniProt
    • Database links

    • Alternative names

      • 40S ribosomal protein S6 antibody
      • Air8 antibody
      • NP33 antibody
      • Phosphoprotein NP33 antibody
      • Pp30 antibody
      • Ribosomal protein S6 antibody
      • RP S6 antibody
      • rps6 antibody
      • RS6 antibody
      • RS6_HUMAN antibody
      • S6 antibody
      • S6 Ribosomal Protein antibody
      see all

    Images

    • Dot Blot analysis of -

      Lane 1: Rb (pS235) phospho peptide

      Lane 2: Rb (pS236) phospho peptide

      Lane 3: Rb (pS235 & pS236) phospho peptide

      Lane 4: Rb non-phospho peptide

      5% NFDM/TBST was used as the diluting and blocking buffer.

      ab97051 Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated was used as the secondary antibody at 1/100000 dilution. Exposure time: 3 minutes.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab80158).

    • ab80158 at 1/100-1/250 dilution staining RPS6 in human breast adenocarcinoma by Immunohistochemistry, Paraffin-embedded tissue.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab80158).

    References

    ab213165 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab213165.
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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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