Overview

  • Product name

    Anti-RPS6 (phospho S235 + S236) antibody
    See all RPS6 primary antibodies
  • Description

    Rabbit polyclonal to RPS6 (phospho S235 + S236)
  • Host species

    Rabbit
  • Specificity

    ab138648 detects endogenous levels of RPS6 only when phosphorylated at S235 + S236
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Synthesized phospho-peptide derived from the internal region of Human RPS6 around the phosphorylation site of S235 + S236.

  • Positive control

    • Jurkat treated with TNF and NIH-3T3 cells treated with PMA.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 49% PBS, 50% Glycerol, 0.88% Sodium chloride
    Note: PBS is without Mg2+ and Ca2+
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab138648 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab138648 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 29 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

References

ab138648 has not yet been referenced specifically in any publications.

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