Key features and details
- Sheep polyclonal to RPS6 (phospho S235 + S236)
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-RPS6 (phospho S235 + S236) antibody
See all RPS6 primary antibodies
DescriptionSheep polyclonal to RPS6 (phospho S235 + S236)
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Chicken, Cow, Xenopus laevis, Cynomolgus monkey
- Raji cells untreated, or treated with 10 µg/ml S6 inhibitor for 30 min.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.50
Preservative: 0.05% Sodium azide
Constituents: 0.238% HEPES, 0.87% Sodium chloride
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab65750 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 2 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 30 kDa).|
FunctionMay play an important role in controlling cell growth and proliferation through the selective translation of particular classes of mRNA.
Sequence similaritiesBelongs to the ribosomal protein S6e family.
modificationsRibosomal protein S6 is the major substrate of protein kinases in eukaryote ribosomes. The phosphorylation is stimulated by growth factors, tumor promoting agents, and mitogens. It is dephosphorylated at growth arrest.
- Information by UniProt
- 40S ribosomal protein S6 antibody
- Air8 antibody
- NP33 antibody
All lanes : Anti-RPS6 (phospho S235 + S236) antibody (ab65750) at 2 µg/ml
Lane 1 : Raji cell extracts, untreated
Lane 2 : Raji cell extracts, treated with 10 µg/ml S6 inhibitor for 30 min
Predicted band size: 30 kDa
Observed band size: 30 kDa
Additional bands at: 48 kDa. We are unsure as to the identity of these extra bands.
ab65750 has not yet been referenced specifically in any publications.