Anti-RPS6 (phospho S240 + S244) antibody (ab65748)

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Western blot - Anti-RPS6 (phospho S240 + S244) antibody (ab65748)

    Key features and details

    • Sheep polyclonal to RPS6 (phospho S240 + S244)
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

    Overview

    • Product name

      Anti-RPS6 (phospho S240 + S244) antibody
      See all RPS6 primary antibodies

    • Description

      Sheep polyclonal to RPS6 (phospho S240 + S244)

    • Host species

      Sheep

    • Tested Applications & Species

      Application Species
      WB
      Human
      See all applications and species data

    • Immunogen

      Synthetic peptide: CSLRASPTSKSPESSQK conjugated to KLH, corresponding to amino acids 236-249 of Human RPS6

      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

    • Positive control

      • Extracts from HEK293 cells, serum starved or 20% new born calf serum treated for 30 min.

    • General notes

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

      One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

      Learn more here.

    Properties

    • Form

      Liquid

    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    • Storage buffer

      pH: 7.50
      Preservative: 0.05% Sodium azide
      Constituents: 0.238% HEPES, 0.87% Sodium chloride
    • Concentration information loading...

    • Purity

      Immunogen affinity purified

    • Clonality

      Polyclonal

    • Isotype

      IgG

    • Research areas

    Associated products

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab65748 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Guaranteed

    Tested applications are guaranteed to work and covered by our Abpromise guarantee.

    Predicted

    Predicted to work for this combination of applications and species but not guaranteed.

    Incompatible

    Does not work for this combination of applications and species.

    Application Species
    WB
    Human
    All applications
    Rat
    Chicken
    Cow
    Xenopus laevis
    Cynomolgus monkey
    Application Abreviews Notes
    WB (1)
    Use a concentration of 2 µg/ml. Detects a band of approximately 32 kDa (predicted molecular weight: 30 kDa).
    Notes
    WB
    Use a concentration of 2 µg/ml. Detects a band of approximately 32 kDa (predicted molecular weight: 30 kDa).

    Target

    Images

    • Western blot - Anti-RPS6 (phospho S240 + S244) antibody (ab65748)
      Western blot - Anti-RPS6 (phospho S240 + S244) antibody (ab65748)
      All lanes : Anti-RPS6 (phospho S240 + S244) antibody (ab65748) at 2 µg/ml

      Lane 1 : HEK293 cell extracts, serum starved
      Lane 2 : HEK293 cell extracts, 20% new born calf serum
      treated for 30 min.

      Predicted band size: 30 kDa
      Observed band size: 32 kDa why is the actual band size different from the predicted?

    References (1)

    Publishing research using ab65748? Please let us know so that we can cite the reference in this datasheet.

    ab65748 has been referenced in 1 publication.

    • Liu Y  et al. Mitochondrial carrier protein overloading and misfolding induce aggresomes and proteostatic adaptations in the cytosol. Mol Biol Cell 30:1272-1284 (2019). PubMed: 30893019

    Customer reviews and Q&As

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    1-2 of 2 Abreviews or Q&A

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-RPS6 (phospho S240 + S244) antibody

     Average
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step
    Thermo Scientific Starting Fisher #PI-37539 Block T20 Blocking Buffer as blocking agent for 10 minute(s) · Concentration: 100% · Temperature: 25°C
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citric Acid
    Sample
    Human Tissue sections (Liver Biopsies)
    Specification
    Liver Biopsies
    Permeabilization
    No
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Nov 14 2013

    Western blot abreview for Anti-RPS6 (phospho S240 + S244) antibody

     Average
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    30 µg
    Gel Running Conditions
    Reduced Denaturing (4-12% Bis-Tris)
    Sample
    Zebrafish Tissue lysate - whole (whole fish)
    Specification
    whole fish
    Treatment
    4% glucose 48hrs 4% fructose 48 hrs
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    Submitted Nov 12 2013

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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