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  1. Link

    rps6-phospho-s240--s244-antibody-ab65748.pdf

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Epigenetics and Nuclear Signaling DNA / RNA Translation Ribosome
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Anti-RPS6 (phospho S240 + S244) antibody (ab65748)

  • Datasheet
Reviews (2) Submit a question References (1)

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Western blot - Anti-RPS6 (phospho S240 + S244) antibody (ab65748)

    Key features and details

    • Sheep polyclonal to RPS6 (phospho S240 + S244)
    • Suitable for: WB
    • Reacts with: Mouse, Human
    • Isotype: IgG

    You may also be interested in

    Protein
    Product image
    Recombinant Human RPS6 protein (ab195250)
    Secondary
    Product image
    Rabbit Anti-Sheep IgG H&L (HRP) (ab6747)

    View more associated products

    Overview

    • Product name

      Anti-RPS6 (phospho S240 + S244) antibody
      See all RPS6 primary antibodies
    • Description

      Sheep polyclonal to RPS6 (phospho S240 + S244)
    • Host species

      Sheep
    • Tested applications

      Suitable for: WBmore details
    • Species reactivity

      Reacts with: Mouse, Human
      Predicted to work with: Rat, Chicken, Cow, Xenopus laevis, Cynomolgus monkey
    • Immunogen

      Synthetic peptide: CSLRASPTSKSPESSQK conjugated to KLH, corresponding to amino acids 236-249 of Human RPS6

      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
    • Positive control

      • Extracts from HEK293 cells, serum starved or 20% new born calf serum treated for 30 min.
    • General notes

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
    • Storage buffer

      pH: 7.50
      Preservative: 0.05% Sodium azide
      Constituents: 0.238% HEPES, 0.87% Sodium chloride
    • Concentration information loading...
    • Purity

      Immunogen affinity purified
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Epigenetics and Nuclear Signaling
      • DNA / RNA
      • Translation
      • Ribosome

    Associated products

    • Isotype control

      • Sheep IgG - Isotype Control (ab37385)
    • Recombinant Protein

      • Recombinant Human RPS6 protein (ab195250)

    Applications

    Our Abpromise guarantee covers the use of ab65748 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB Use a concentration of 2 µg/ml. Detects a band of approximately 32 kDa (predicted molecular weight: 30 kDa).

    Target

    • Function

      May play an important role in controlling cell growth and proliferation through the selective translation of particular classes of mRNA.
    • Sequence similarities

      Belongs to the ribosomal protein S6e family.
    • Post-translational
      modifications

      Ribosomal protein S6 is the major substrate of protein kinases in eukaryote ribosomes. The phosphorylation is stimulated by growth factors, tumor promoting agents, and mitogens. It is dephosphorylated at growth arrest.
    • Target information above from: UniProt accession P62753 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 396148 Chicken
      • Entrez Gene: 787914 Cow
      • Entrez Gene: 6194 Human
      • Entrez Gene: 20104 Mouse
      • Entrez Gene: 667739 Mouse
      • Entrez Gene: 29304 Rat
      • Entrez Gene: 380281 Xenopus laevis
      • Omim: 180460 Human
      • SwissProt: P47838 Chicken
      • SwissProt: Q5E995 Cow
      • SwissProt: Q4R4K6 Cynomolgus monkey
      • SwissProt: P62753 Human
      • SwissProt: P62754 Mouse
      • SwissProt: P62755 Rat
      • SwissProt: P39017 Xenopus laevis
      • Unigene: 408073 Human
      • Unigene: 379007 Mouse
      • Unigene: 425090 Mouse
      • Unigene: 34341 Rat
      • Unigene: 53779 Xenopus laevis
      see all
    • Alternative names

      • 40S ribosomal protein S6 antibody
      • Air8 antibody
      • NP33 antibody
      • Phosphoprotein NP33 antibody
      • Pp30 antibody
      • Ribosomal protein S6 antibody
      • RP S6 antibody
      • rps6 antibody
      • RS6 antibody
      • RS6_HUMAN antibody
      • S6 antibody
      • S6 Ribosomal Protein antibody
      see all

    Images

    • Western blot - Anti-RPS6 (phospho S240 + S244) antibody (ab65748)
      Western blot - Anti-RPS6 (phospho S240 + S244) antibody (ab65748)
      All lanes : Anti-RPS6 (phospho S240 + S244) antibody (ab65748) at 2 µg/ml

      Lane 1 : HEK293 cell extracts, serum starved
      Lane 2 : HEK293 cell extracts, 20% new born calf serum
      treated for 30 min.

      Predicted band size: 30 kDa
      Observed band size: 32 kDa
      why is the actual band size different from the predicted?

    Protocols

    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (1)

    Publishing research using ab65748? Please let us know so that we can cite the reference in this datasheet.

    ab65748 has been referenced in 1 publication.

    • Liu Y  et al. Mitochondrial carrier protein overloading and misfolding induce aggresomes and proteostatic adaptations in the cytosol. Mol Biol Cell 30:1272-1284 (2019). PubMed: 30893019

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-2 of 2 Abreviews or Q&A

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-RPS6 (phospho S240 + S244) antibody

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step
    Thermo Scientific Starting Fisher #PI-37539 Block T20 Blocking Buffer as blocking agent for 10 minute(s) · Concentration: 100% · Temperature: 25°C
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citric Acid
    Sample
    Human Tissue sections (Liver Biopsies)
    Specification
    Liver Biopsies
    Permeabilization
    No
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Nov 14 2013

    Western blot abreview for Anti-RPS6 (phospho S240 + S244) antibody

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    30 µg
    Gel Running Conditions
    Reduced Denaturing (4-12% Bis-Tris)
    Sample
    Zebrafish Tissue lysate - whole (whole fish)
    Specification
    whole fish
    Treatment
    4% glucose 48hrs 4% fructose 48 hrs
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    Submitted Nov 12 2013

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