Key features and details
- Sheep polyclonal to RPS6 (phospho S240 + S244)
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-RPS6 (phospho S240 + S244) antibody
See all RPS6 primary antibodies
DescriptionSheep polyclonal to RPS6 (phospho S240 + S244)
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Chicken, Cow, Xenopus laevis, Cynomolgus monkey
- Extracts from HEK293 cells, serum starved or 20% new born calf serum treated for 30 min.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.50
Preservative: 0.05% Sodium azide
Constituents: 0.238% HEPES, 0.87% Sodium chloride
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab65748 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 2 µg/ml. Detects a band of approximately 32 kDa (predicted molecular weight: 30 kDa).|
FunctionMay play an important role in controlling cell growth and proliferation through the selective translation of particular classes of mRNA.
Sequence similaritiesBelongs to the ribosomal protein S6e family.
modificationsRibosomal protein S6 is the major substrate of protein kinases in eukaryote ribosomes. The phosphorylation is stimulated by growth factors, tumor promoting agents, and mitogens. It is dephosphorylated at growth arrest.
- Information by UniProt
- 40S ribosomal protein S6 antibody
- Air8 antibody
- NP33 antibody
All lanes : Anti-RPS6 (phospho S240 + S244) antibody (ab65748) at 2 µg/ml
Lane 1 : HEK293 cell extracts, serum starved
Lane 2 : HEK293 cell extracts, 20% new born calf serum
treated for 30 min.
Predicted band size: 30 kDa
Observed band size: 32 kDa why is the actual band size different from the predicted?
ab65748 has been referenced in 1 publication.
- Liu Y et al. Mitochondrial carrier protein overloading and misfolding induce aggresomes and proteostatic adaptations in the cytosol. Mol Biol Cell 30:1272-1284 (2019). PubMed: 30893019