Anti-RPS6 (phospho S240 + S244) antibody (ab65748)
Key features and details
- Sheep polyclonal to RPS6 (phospho S240 + S244)
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-RPS6 (phospho S240 + S244) antibody
See all RPS6 primary antibodies -
Description
Sheep polyclonal to RPS6 (phospho S240 + S244) -
Host species
Sheep -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Chicken, Cow, Xenopus laevis, Cynomolgus monkey
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Immunogen
Synthetic peptide: CSLRASPTSKSPESSQK conjugated to KLH, corresponding to amino acids 236-249 of Human RPS6
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Positive control
- Extracts from HEK293 cells, serum starved or 20% new born calf serum treated for 30 min.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.50
Preservative: 0.05% Sodium azide
Constituents: 0.238% HEPES, 0.87% Sodium chloride -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab65748 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 2 µg/ml. Detects a band of approximately 32 kDa (predicted molecular weight: 30 kDa). |
Target
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Function
May play an important role in controlling cell growth and proliferation through the selective translation of particular classes of mRNA. -
Sequence similarities
Belongs to the ribosomal protein S6e family. -
Post-translational
modificationsRibosomal protein S6 is the major substrate of protein kinases in eukaryote ribosomes. The phosphorylation is stimulated by growth factors, tumor promoting agents, and mitogens. It is dephosphorylated at growth arrest. - Information by UniProt
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Database links
- Entrez Gene: 396148 Chicken
- Entrez Gene: 787914 Cow
- Entrez Gene: 6194 Human
- Entrez Gene: 20104 Mouse
- Entrez Gene: 667739 Mouse
- Entrez Gene: 29304 Rat
- Entrez Gene: 380281 Xenopus laevis
- Omim: 180460 Human
see all -
Alternative names
- 40S ribosomal protein S6 antibody
- Air8 antibody
- NP33 antibody
see all
Images
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Western blot - Anti-RPS6 (phospho S240 + S244) antibody (ab65748)All lanes : Anti-RPS6 (phospho S240 + S244) antibody (ab65748) at 2 µg/ml
Lane 1 : HEK293 cell extracts, serum starved
Lane 2 : HEK293 cell extracts, 20% new born calf serum
treated for 30 min.
Predicted band size: 30 kDa
Observed band size: 32 kDa why is the actual band size different from the predicted?
Datasheets and documents
References (1)
ab65748 has been referenced in 1 publication.
- Liu Y et al. Mitochondrial carrier protein overloading and misfolding induce aggresomes and proteostatic adaptations in the cytosol. Mol Biol Cell 30:1272-1284 (2019). PubMed: 30893019
