Overview

  • Product name
    Anti-RPS6 (phospho S244 + S247) antibody
    See all RPS6 primary antibodies
  • Description
    Rabbit polyclonal to RPS6 (phospho S244 + S247)
  • Host species
    Rabbit
  • Specificity
    This antibody does not cross react with RPS6 phosphorylated on serines 235 and 236.
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide derived from the region of human RPS6 that contains serines 244 and 247 (unfortunately, the amino acid sequence is considered to be commercially sensitive)

  • Positive control
    • HeLa +/- TNF-alpha or anisomycin
  • General notes
    Centrifuge before opening to settle contents.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.30
    Preservative: 0.05% Sodium azide
    Constituents: PBS, 50% Glycerol, 0.1% BSA

    Dulbecco's PBS without magnesium and calcium
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Purified from rabbit serum by sequential epitope-specific chromatography. The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated RPS6. The final product is generated by affinity chromatography using a RPS6-derived peptide that is phosphorylated at serines 244 and 247.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab12865 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 32 kDa (predicted molecular weight: 31 kDa).

Target

Images

  • Lysates prepared from HeLa cells left untreated (1) or treated with TNF-alpha (2-5) were resolved by SDS-PAGE on a 14% polyacrylamide gel and transferred to PVDF. Membranes were blocked with a 5% BSA-TBST buffer for one hour at room temperature, and incubated with ab12865 for two hours at room temperature in a 3% BSA-TBST buffer, following prior incubation with: no peptide (1, 2), the non-phosphopeptide corresponding to the immunogen (3), a generic phosphoserine-containing peptide (4), or the phosphopeptide immunogen (5). After washing, membranes were incubated with goat anti-rabbit IgG HRP conjugate and bands were detected. The data show that only the peptide corresponding to RPS6 [phospho S244 & phospho S247] blocks the signal, verifying the specificity of the antibody.
    Lysates prepared from HeLa cells left untreated (1) or treated with TNF-alpha (2-5) were resolved by SDS-PAGE on a 14% polyacrylamide gel and transferred to PVDF. Membranes were blocked with a 5% BSA-TBST

References

This product has been referenced in:
  • Järås M  et al. Csnk1a1 inhibition has p53-dependent therapeutic efficacy in acute myeloid leukemia. J Exp Med 211:605-12 (2014). WB ; Human . Read more (PubMed: 24616378) »
See 1 Publication for this product

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab12865.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up