Key features and details
- Rabbit polyclonal to RPS6KC1
- Suitable for: IHC-P, ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-RPS6KC1 antibody
DescriptionRabbit polyclonal to RPS6KC1
Tested applicationsSuitable for: IHC-P, ICC/IF, WBmore details
Species reactivityReacts with: Human
- WB: RPS6KC1 over-expression - HEK-293T whole cell lysates; IHC-P: Human duodenum tissue. ICC: A431 cells.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab224469 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100.
|WB||Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 119 kDa.|
FunctionMay be involved in transmitting sphingosine-1 phosphate (SPP)-mediated signaling into the cell.
Tissue specificityHighly expressed in testis, skeletal muscle, brain, heart, placenta, kidney and liver and weakly expressed in thymus, small intestine, lung and colon.
Sequence similaritiesBelongs to the protein kinase superfamily. Ser/Thr protein kinase family. S6 kinase subfamily.
Contains 1 MIT domain.
Contains 2 protein kinase domains.
Contains 1 PX (phox homology) domain.
DomainThe first protein kinase domain appears to be a pseudokinase domain as it does not contain the classical characteristics, such as the ATP-binding motif, ATP-binding site and active site.
Cellular localizationCytoplasm. Membrane. Also found in some small dot-like or ring-shaped early endosome structures.
- Information by UniProt
- 52 kDa ribosomal protein S6 kinase antibody
- humS6PKh1 antibody
- KS6C1_HUMAN antibody
All lanes : Anti-RPS6KC1 antibody (ab224469) at 0.4 µg/ml
Lane 1 : Control (only vetor transfected) - HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : RPS6KC1 over-expression - HEK-293T whole cell lysate
Predicted band size: 119 kDa
Immunohistochemical analysis of human duodenum tissue labeling RPS6KC1 in the cytoplasm of glandular cells with ab224469 at a 1/20 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunofluorescent analysis of A431 (human epidermoid carcinoma cell line) cells labeling RPS6KC1 (green) in the vesicles with ab224469 at 2 µg/ml.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab224469 has not yet been referenced specifically in any publications.