Key features and details
- Rabbit polyclonal to RPS7
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-RPS7 antibody
DescriptionRabbit polyclonal to RPS7
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Rat, Human
Predicted to work with: Cow, Cat, Zebrafish
Recombinant full length protein corresponding to Human RPS7 aa 1-194.
MFSSSAKIVKPNGEKPDEFESGISQALLELEMNSDLKAQLRELNITAAKE IEVGGGRKAIIIFVPVPQLKSFQKIQVRLVRELEKKFSGKHVVFIAQRRI LPKPTRKSRTKNKQKRPRSRTLTAVHDAILEDLVFPSEIVGKRIRVKLDG SRLIKVHLDKAQQNNVEHKVETFSGVYKKLTGKDVNFEFPEFQL
Database link: P62081
- WB: HeLa, SW480, NIH/3T3 and MCF7 cell extracts; mouse and rat liver extracts. IHC-P: Human small intestine and cerebellum tissue.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Concentration information loading...
Our Abpromise guarantee covers the use of ab230862 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/2000. Predicted molecular weight: 22 kDa.|
|IHC-P||1/50 - 1/200.|
FunctionRequired for rRNA maturation.
Involvement in diseaseDefects in RPS7 are the cause of Diamond-Blackfan anemia type 8 (DBA8) [MIM:612563]. DBA8 is a form of Diamond-Blackfan anemia, a congenital non-regenerative hypoplastic anemia that usually presents early in infancy. Diamond-Blackfan anemia is characterized by a moderate to severe macrocytic anemia, erythroblastopenia, and an increased risk of malignancy. 30 to 40% of Diamond-Blackfan anemia patients present with short stature and congenital anomalies, the most frequent being craniofacial (Pierre-Robin syndrome and cleft palate), thumb and urogenital anomalies.
Sequence similaritiesBelongs to the ribosomal protein S7e family.
- Information by UniProt
- 40S ribosomal protein S7 antibody
- DBA8 antibody
- Ribosomal protein S7 antibody
All lanes : Anti-RPS7 antibody (ab230862) at 1/500 dilution
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell extract
Lane 2 : NIH/3T3 (mouse embryo fibroblast cell line) cell extract
Lane 3 : SW480 (human colorectal adenocarcinoma cell line) cell extract
Lane 4 : MCF7 (human breast adenocarcinoma cell line) cell extract
Lane 5 : A549 (human lung carcinoma cell line) cell extract
Lane 6 : Mouse liver extract
Lane 7 : Rat liver extract
Developed using the ECL technique.
Predicted band size: 22 kDa
Formalin-fixed, paraffin-embedded human cerebellum tissue stained for RPS7 with Immunohistochemistry at 1/50 dilution in immunohistochemical analysis.
Formalin-fixed, paraffin-embedded human small intestine tissue stained for RPS7 with Immunohistochemistry at 1/50 dilution in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab230862 has not yet been referenced specifically in any publications.