• Product name
  • Description
    Mouse monoclonal to RPS7
  • Host species
  • Tested applications
    Suitable for: WB, IHC-P, Flow Cyt, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen


  • Positive control
    • IHC-P: FFPE human breast adenocarcinoma tissue sections.
  • General notes

    This product was changed from ascites to tissue culture supernatant on 30th April 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.


  • Form
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: None
    PBS, pH 7.2
  • Purity
    Tissue culture supernatant
  • Purification notes
    Purified from TCS.
  • Clonality
  • Isotype
  • Light chain type
  • Research areas


Our Abpromise guarantee covers the use of ab57637 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 22 kDa.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt Use at an assay dependent concentration.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.


ICC/IF Use at an assay dependent concentration.


  • Function
    Required for rRNA maturation.
  • Involvement in disease
    Defects in RPS7 are the cause of Diamond-Blackfan anemia type 8 (DBA8) [MIM:612563]. DBA8 is a form of Diamond-Blackfan anemia, a congenital non-regenerative hypoplastic anemia that usually presents early in infancy. Diamond-Blackfan anemia is characterized by a moderate to severe macrocytic anemia, erythroblastopenia, and an increased risk of malignancy. 30 to 40% of Diamond-Blackfan anemia patients present with short stature and congenital anomalies, the most frequent being craniofacial (Pierre-Robin syndrome and cleft palate), thumb and urogenital anomalies.
  • Sequence similarities
    Belongs to the ribosomal protein S7e family.
  • Information by UniProt
  • Database links
  • Alternative names
    • 40S ribosomal protein S7 antibody
    • DBA8 antibody
    • Ribosomal protein S7 antibody
    • RPS 7 antibody
    • rps7 antibody
    • RS7_HUMAN antibody
    • S7 antibody
    see all


  • RPS7 antibody (ab57637) at 1ug/lane + HeLa cell lysate at 25ug/lane.

    This image was generated using the ascites version of the product.

  • IHC image of RPS7 staining in human breast adenocarcinoma formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab57637, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    This image was generated using the ascites version of the product.

  • ICC/IF image of ab57637 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab57637, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879 Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    This image was generated using the ascites version of the product.

  • Overlay histogram showing HeLa cells stained with ab57637 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab57637, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

    This image was generated using the ascites version of the product.


This product has been referenced in:
  • Anton H  et al. Investigating the cellular distribution and interactions of HIV-1 nucleocapsid protein by quantitative fluorescence microscopy. PLoS One 10:e0116921 (2015). Read more (PubMed: 25723396) »
  • Miettinen TP & Björklund M Modified ribosome profiling reveals high abundance of ribosome protected mRNA fragments derived from 3' untranslated regions. Nucleic Acids Res 43:1019-34 (2015). Read more (PubMed: 25550424) »
See all 2 Publications for this product

Customer reviews and Q&As


I am very pleased to hear you would like to test ab57637 and ab58328 in Xenopus. I have performed the sequence alignment of the immunogen used to raise these antibodies to that of the most similar Xenopus sequence. Ab57637 and ab58328 matched by 97 and 80% homology respectively. We typically find that homologies are likely (but not guaranteed) to show cross reactivity. The score with ab58328 lower than this and I am therefore not confident that the test is likely to work, however as with this is monoclonal it may be that you are lucky and the epitope recognized is within a region of similarity. I have generated the two testing discount codes as below:
ab57637 DISCOUNT CODE: xxxxxxxx Expiration date: 2012-08-22
ab58328 DISCOUNT CODE: xxxxxxxx Expiration date: 2012-08-22
These codes will give you: 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview for Xenopus and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site:
Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways:
1) Call to place your order and mention the code to our customer service department;
2) Include the code in your fax order;
3) Place your order on the web and enter the promotional code.
Any feedback that you can provide will be greatly appreciated, whether positive or negative. The code must be used to purchase your free primary antibody within the expire date above. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.
The terms and conditions applicable to this offer can be found here:

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