Key features and details
- Rabbit polyclonal to RPS9
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-RPS9 antibody
See all RPS9 primary antibodies
DescriptionRabbit polyclonal to RPS9
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide derived from an internal region of human RPS9
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 0.87% Sodium chloride, PBS
Without Mg2+ and Ca2+
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab74711 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Predicted molecular weight: 26 kDa.|
|ICC/IF||1/500 - 1/1000.|
Sequence similaritiesBelongs to the ribosomal protein S4P family.
Contains 1 S4 RNA-binding domain.
Cellular localizationCytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs.
- Information by UniProt
- 40S ribosomal protein S9 antibody
- OTTHUMP00000069577 antibody
- OTTHUMP00000069584 antibody
All lanes : Anti-RPS9 antibody (ab74711) at 1/500 dilution
Lane 1 : extracts from HepG2 cells at 20 µg
Lane 2 : extracts from COLO
cells at 20 µg
Lane 3 : extracts from HT-29 cells at 20 µg
Lane 4 : extracts from HeLa cells at 30 µg
Lane 5 : extracts from HUVEC cells at 20 µg
Lane 6 : extracts from HepG2 cells at 20 µg with immunizing peptide at 10 µg
Predicted band size: 26 kDa
Observed band size: 26 kDa
Immunofluorescence analysis of A549 cells, using ab74711 at 1/500-1/1000 dilution in the presence (+) or absence (-) of immunising peptide.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab74711 has been referenced in 3 publications.
- Iizumi Y et al. The flavonoid apigenin downregulates CDK1 by directly targeting ribosomal protein S9. PLoS One 8:e73219 (2013). WB, Flow Cyt . PubMed: 24009741
- Gallo S et al. Tagging of functional ribosomes in living cells by HaloTag® technology. In Vitro Cell Dev Biol Anim 47:132-8 (2011). WB ; Human . PubMed: 21082278
- Yang PY et al. Activity-based proteome profiling of potential cellular targets of Orlistat--an FDA-approved drug with anti-tumor activities. J Am Chem Soc 132:656-66 (2010). PubMed: 20028024