Anti-Rpt5/TBP-1 antibody (ab22676)

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Overview

  • Product name

    Anti-Rpt5/TBP-1 antibody

  • Description

    Rabbit polyclonal to Rpt5/TBP-1

  • Host species

    Rabbit

  • Specificity

    ab22676 reacts with 19S regulator ATPase subunit Rpt5/TBP-1 (Yta1).

  • Tested applications

    Suitable for: WBmore details

  • Species reactivity

    Reacts with: Saccharomyces cerevisiae, Arabidopsis thaliana, Fungi

  • Immunogen

    Recombinant fragment corresponding to Saccharomyces cerevisiae Rpt5/TBP-1 aa 1-100 (N terminal).
    Sequence:

    MATLEELDAQTLPGDDELDQEILNLSTQELQTRAKLLDNEIRIFRSELQR LSHENNVMLEKIKDNKEKIKNNRQLPYLVANVVEVMDMNEIEDKENSEST

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • Yeast 26S proteasome

  • General notes

     This product was previously labelled as Rpt5

     

Properties

Applications

Our Abpromise guarantee covers the use of ab22676 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notes
    WB: 1/10000 - 1/50000. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa).
    Notes: Dilute to working strength with phosphate buffered saline pH 7.2-7.4 and 1% normal goat serum (if a goat anti-rabbit IgG linker antibody is to be used).

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Target

    • Relevance

      In the yeast S. cerevisiae 12 different members of a novel gene family of putative ATPases have been identified and characterised. Due to their similarities to TBP1, a human immunodeficiency tat binding protein, they have been designated the YTA family or yeast tat binding analogues. All share a region of high similarity of approximately 300 amino acids in length. Rpt5p or YTA1 ,is one of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates.

    • Cellular localization

      Nuclear

    • Database links

      • Alternative names

        • 26S protease regulatory subunit 6A antibody
        • TAT binding protein homolog 1 antibody
        • TBP1 antibody
        • YTA1 antibody

      Images

      • Western blot - Anti-Rpt5/TBP-1 antibody (ab22676)
        Western blot - Anti-Rpt5/TBP-1 antibody (ab22676)
        Anti-Rpt5/TBP-1 antibody (ab22676) at 1/25000 dilution (PVDF blotting membrane)
        Developed using the ECL technique.

        Performed under reducing conditions.

        Predicted band size: 48 kDa
        Observed band size: 48 kDa


        Exposure time: 1 minute

      References

      Publishing research using ab22676? Please let us know so that we can cite the reference in this datasheet.

      ab22676 has been referenced in 2 publications.

      • Song Z  et al. Constitutive Expression of miR408 Improves Biomass and Seed Yield in Arabidopsis. Front Plant Sci 8:2114 (2017). PubMed: 29422907
      • Wang Y  et al. Repression of MYBL2 by Both microRNA858a and HY5 Leads to the Activation of Anthocyanin Biosynthetic Pathway in Arabidopsis. Mol Plant 9:1395-1405 (2016). PubMed: 27450422

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