ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
RPUSD3 belongs to the pseudouridine synthase rluA family. Members of this family are involved in modifying bases in RNA molecules. Pseudouridine synthases are responsible for synthesis of pseudouridine from uracil.
Immunofluorescence analysis of SH-SY5Y cells (fixative 4% paraformaldehyde) labeling RPUSD3 with ab181121 at a 1/250 dilution. Goat anti rabbit IgG (Alexa Fluor® 555) secondary used at a 1/200 diution.
Western blot - Anti-RPUSD3 antibody [EPR12911] (ab181121)
All lanes : Anti-RPUSD3 antibody [EPR12911] (ab181121) at 1/1000 dilution
Lane 1 : U87-MG lysate Lane 2 : SH-SY5Y lysate Lane 3 : 293T lysate Lane 4 : Human fetal brain lysate
Lysates/proteins at 20 µg per lane.
Secondary All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution