Anti-RPUSD4 antibody (ab122571)
- Datasheet
- References (1)
- Protocols
Overview
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Product name
Anti-RPUSD4 antibody -
Description
Rabbit polyclonal to RPUSD4 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
antigen sequence: NLVVINKPYG LPVHGGPGVQ LCITDVLPIL AKMLHGHKAE PLHLCHRLDK ETTGVMVLAW DKDMAHQVQE LFRTRQVVKK YWAITVHVP, corresponding to internal sequence amino acids 105-193 of Human RPUSD4.
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Positive control
- Human colon tissue; RPUSD4 over-expression HEK293T lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C or -80°C. Avoid freeze / thaw cycle. Store undiluted. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab122571 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| WB | 1/250 - 1/500. | |
| ICC/IF | Use a concentration of 1 - 4 µg/ml. Recommend PFA Fixation and Triton X-100 treatment |
Target
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Sequence similarities
Belongs to the pseudouridine synthase rluA family. - Information by UniProt
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Database links
- Entrez Gene: 84881 Human
- SwissProt: Q96CM3 Human
- Unigene: 150458 Human
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Alternative names
- RNA pseudouridylate synthase domain-containing protein 4 antibody
- rpusd4 antibody
- RUSD4_HUMAN antibody
Images
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Immunocytochemistry/ Immunofluorescence - Anti-RPUSD4 antibody (ab122571)Immunofluorescent staining of Human cell line A-431 shows positivity in nucleus but not nucleoli and mitochondria. Recommended concentration of ab122571 1-4 µg/ml. Cells treated with PFA/Triton X-100. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPUSD4 antibody (ab122571)ab122571, at 1/50 dilution, staining RPUSD4 in paraffin-embedded Human colon tissue by Immunohistochemistry. -
Western blot - Anti-RPUSD4 antibody (ab122571)All lanes : Anti-RPUSD4 antibody (ab122571) at 1/250 dilution
Lane 1 : Vector only transfected HEK293T cell lysate
Lane 2 : RPUSD4 overexpression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in HEK293T cells
Developed using the ECL technique.
Protocols
Datasheets and documents
References
This product has been referenced in:
- Zaganelli S et al. The Pseudouridine Synthase RPUSD4 Is an Essential Component of Mitochondrial RNA Granules. J Biol Chem 292:4519-4532 (2017). Read more (PubMed: 28082677) »
