Anti-RPUSD4 antibody (ab122571)
Key features and details
- Rabbit polyclonal to RPUSD4
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-RPUSD4 antibody -
Description
Rabbit polyclonal to RPUSD4 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
antigen sequence: NLVVINKPYG LPVHGGPGVQ LCITDVLPIL AKMLHGHKAE PLHLCHRLDK ETTGVMVLAW DKDMAHQVQE LFRTRQVVKK YWAITVHVP, corresponding to internal sequence amino acids 105-193 of Human RPUSD4.
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Positive control
- WB: Human colon tissue; RPUSD4 over-expression HEK293T lysate. IHC: Human skin tissue
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C or -80°C. Avoid freeze / thaw cycle. Store undiluted. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab122571 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| WB | Use a concentration of 0.04 - 0.4 µg/ml. |
Target
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Sequence similarities
Belongs to the pseudouridine synthase rluA family. - Information by UniProt
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Database links
- Entrez Gene: 84881 Human
- SwissProt: Q96CM3 Human
- Unigene: 150458 Human
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Alternative names
- RNA pseudouridylate synthase domain-containing protein 4 antibody
- rpusd4 antibody
- RUSD4_HUMAN antibody
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPUSD4 antibody (ab122571)
ab122571, at 1/50 dilution, staining RPUSD4 in paraffin-embedded Human skin tissue by Immunohistochemistry.
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Western blot - Anti-RPUSD4 antibody (ab122571)All lanes : Anti-RPUSD4 antibody (ab122571) at 1/250 dilution
Lane 1 : Vector only transfected HEK293T cell lysate
Lane 2 : RPUSD4 overexpression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in HEK293T cells
Developed using the ECL technique.
Protocols
Datasheets and documents
References (1)
ab122571 has been referenced in 1 publication.
- Zaganelli S et al. The Pseudouridine Synthase RPUSD4 Is an Essential Component of Mitochondrial RNA Granules. J Biol Chem 292:4519-4532 (2017). PubMed: 28082677
