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Rabbit polyclonal to RPUSD4
antigen sequence: NLVVINKPYG LPVHGGPGVQ LCITDVLPIL AKMLHGHKAE PLHLCHRLDK ETTGVMVLAW DKDMAHQVQE LFRTRQVVKK YWAITVHVP, corresponding to internal sequence amino acids 105-193 of Human RPUSD4.
- Human colon tissue; RPUSD4 over-expression HEK293T lysate.
Shipped at 4°C. Store at -20°C or -80°C. Avoid freeze / thaw cycle. Store undiluted.
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
Concentration information loading...
Immunogen affinity purified
Our Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
||1/250 - 1/500.
||Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
Belongs to the pseudouridine synthase rluA family.
Information by UniProt
- RNA pseudouridylate synthase domain-containing protein 4 antibody
- rpusd4 antibody
- RUSD4_HUMAN antibody
Immunocytochemistry/ Immunofluorescence - Anti-RPUSD4 antibody (ab122571)
Immunofluorescent staining of Human cell line A-431 shows positivity in nucleus but not nucleoli and mitochondria. Recommended concentration of ab122571 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPUSD4 antibody (ab122571)
ab122571, at 1/50 dilution, staining RPUSD4 in paraffin-embedded Human colon tissue by Immunohistochemistry.
Western blot - Anti-RPUSD4 antibody (ab122571)
All lanes : Anti-RPUSD4 antibody (ab122571) at 1/250 dilution
Lane 1 : Vector only transfected HEK293T cell lysate
Lane 2 : RPUSD4 overexpression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in HEK293T cells
Developed using the ECL technique.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"