Key features and details
- Rabbit polyclonal to RPUSD4
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-RPUSD4 antibody
DescriptionRabbit polyclonal to RPUSD4
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Human
- WB: Human colon tissue; RPUSD4 over-expression HEK293T lysate. IHC: Human skin tissue
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at -20°C or -80°C. Avoid freeze / thaw cycle. Store undiluted.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab122571 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||Use a concentration of 0.04 - 0.4 µg/ml.|
Sequence similaritiesBelongs to the pseudouridine synthase rluA family.
- Information by UniProt
- RNA pseudouridylate synthase domain-containing protein 4 antibody
- rpusd4 antibody
- RUSD4_HUMAN antibody
ab122571, at 1/50 dilution, staining RPUSD4 in paraffin-embedded Human skin tissue by Immunohistochemistry.
All lanes : Anti-RPUSD4 antibody (ab122571) at 1/250 dilution
Lane 1 : Vector only transfected HEK293T cell lysate
Lane 2 : RPUSD4 overexpression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in HEK293T cells
Developed using the ECL technique.
ab122571 has been referenced in 1 publication.
- Zaganelli S et al. The Pseudouridine Synthase RPUSD4 Is an Essential Component of Mitochondrial RNA Granules. J Biol Chem 292:4519-4532 (2017). PubMed: 28082677