Overview

  • Product name

  • Description

    Goat polyclonal to RRAD
  • Host species

    Goat
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse
    Predicted to work with: Rat, Rabbit, Cow, Dog, Human, Pig
  • Immunogen

    Synthetic peptide:

    C-HRRSMPVDERDLQ

    , corresponding to internal sequence amino acids 36-48 of Human RRAD (NP_004156.1).

  • Positive control

    • Mouse Heart lysates.
  • General notes

    Reported variants represent identical protein: NP_004156.1, NP_001122322.1.

Properties

Applications

Our Abpromise guarantee covers the use of ab136865 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 33 kDa).

Target

  • Function

    May play an important role in cardiac antiarrhythmia via the strong suppression of voltage-gated L-type Ca(2+) currents. Regulates voltage-dependent L-type calcium channel subunit alpha-1C trafficking to the cell membrane (By similarity). Inhibits cardiac hypertrophy through the calmodulin-dependent kinase II (CaMKII) pathway. Inhibits phosphorylation and activation of CAMK2D.
  • Tissue specificity

    Most abundantly expressed in the heart. Also found in the skeletal muscle and lung. Lesser amounts in placenta and kidney. Also detected in adipose tissue. Overexpressed in muscle of type II diabetic humans.
  • Sequence similarities

    Belongs to the small GTPase superfamily. RGK family.
  • Cellular localization

    Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • GTP binding protein RAD antibody
    • GTP-binding protein RAD antibody
    • RAD antibody
    • RAD_HUMAN antibody
    • RAD1 antibody
    • RAS (RAD and GEM) like GTP binding 3 antibody
    • Ras associated with diabetes antibody
    • Ras related associated with diabetes antibody
    • REM3 antibody
    • RRAD antibody
    see all

Images

  • All lanes : Anti-RRAD antibody (ab136865) at 0.5 µg/ml

    Lane 1 : HEK293 lysates overexpressing HA-tagged Mouse GTPase EV
    Lane 2 : HEK293 lysates overexpressing HA-tagged Mouse GTPase Rem
    Lane 3 : HEK293 lysates overexpressing HA-tagged Mouse GTPase Rem2
    Lane 4 : HEK293 lysates overexpressing HA-tagged Mouse GTPase RRAD
    Lane 5 : HEK293 lysates overexpressing HA-tagged Mouse GTPase Gem

    Lysates/proteins at 10 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 33 kDa

  • All lanes : anti-HA antibody

    Lane 1 : HEK293 lysates overexpressing HA-tagged Mouse GTPase EV
    Lane 2 : HEK293 lysates overexpressing HA-tagged Mouse GTPase Rem
    Lane 3 : HEK293 lysates overexpressing HA-tagged Mouse GTPase Rem2
    Lane 4 : HEK293 lysates overexpressing HA-tagged Mouse GTPase RRAD
    Lane 5 : HEK293 lysates overexpressing HA-tagged Mouse GTPase Gem

    Predicted band size: 33 kDa

  • All lanes : Anti-RRAD antibody (ab136865) at 0.5 µg/ml

    Lane 1 : WT mouse heart tissue lysate
    Lane 2 : KO mouse heart tissue lysate

    Lysates/proteins at 100 µg per lane.

    Predicted band size: 33 kDa

References

ab136865 has not yet been referenced specifically in any publications.

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