Overview

  • Product name

    Anti-RRAD antibody [EPR12856]
    See all RRAD primary antibodies
  • Description

    Rabbit monoclonal [EPR12856] to RRAD
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human RRAD aa 50-150 (Cysteine residue). The exact sequence is proprietary.
    Database link: P55042

  • Positive control

    • WB: Human fetal heart, fetal lung and A673 lysates.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab177151 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 33 kDa.
  • Application notes
    Is unsuitable for IP.
  • Target

    • Function

      May play an important role in cardiac antiarrhythmia via the strong suppression of voltage-gated L-type Ca(2+) currents. Regulates voltage-dependent L-type calcium channel subunit alpha-1C trafficking to the cell membrane (By similarity). Inhibits cardiac hypertrophy through the calmodulin-dependent kinase II (CaMKII) pathway. Inhibits phosphorylation and activation of CAMK2D.
    • Tissue specificity

      Most abundantly expressed in the heart. Also found in the skeletal muscle and lung. Lesser amounts in placenta and kidney. Also detected in adipose tissue. Overexpressed in muscle of type II diabetic humans.
    • Sequence similarities

      Belongs to the small GTPase superfamily. RGK family.
    • Cellular localization

      Cell membrane.
    • Information by UniProt
    • Database links

    • Alternative names

      • GTP binding protein RAD antibody
      • GTP-binding protein RAD antibody
      • RAD antibody
      • RAD_HUMAN antibody
      • RAD1 antibody
      • RAS (RAD and GEM) like GTP binding 3 antibody
      • Ras associated with diabetes antibody
      • Ras related associated with diabetes antibody
      • REM3 antibody
      • RRAD antibody
      see all

    Images

    • Anti-RRAD antibody [EPR12856] (ab177151) at 1/5000 dilution (Purified) + Human fetal lung lysates at 15 µg

      Secondary
      Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

      Predicted band size: 33 kDa
      Observed band size: 33 kDa

    • Anti-RRAD antibody [EPR12856] (ab177151) at 1/5000 dilution (Purified) + A-673 (Human muscle Ewing's Sarcoma) whole cell lysates at 15 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/2000 dilution

      Predicted band size: 33 kDa
      Observed band size: 33 kDa



      We are unsure how to define the extra bands.

    • All lanes : Anti-RRAD antibody [EPR12856] (ab177151) at 1/1000 dilution (unpurified)

      Lane 1 : Human fetal heart lysate
      Lane 2 : Human fetal lung lysate
      Lane 3 : A673 lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 33 kDa

    References

    ab177151 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab177151.
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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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