Key features and details
- Rabbit polyclonal to RRAGC
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-RRAGC antibody
See all RRAGC primary antibodies
DescriptionRabbit polyclonal to RRAGC
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Sheep, Guinea pig, Dog, Pig, Rhesus monkey, Gorilla, Orangutan, Bat
- 293T, HeLa, Jurkat, mouse TCMK-1 and mouse NIH3T3 whole cell lysates.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab168819 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 44 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
FunctionHas guanine nucleotide-binding activity but weak intrinsic GTPase activity. Probably required for the amino acid-induced relocalization of mTORC1 to the lysosomes and its subsequent activation by the GTPase RHEB. This is a crucial step in the activation of the TOR signaling cascade by amino acids.
Sequence similaritiesBelongs to the GTR/RAG GTP-binding protein family.
Cellular localizationCytoplasm. Nucleus. Lysosome. Predominantly cytoplasmic. May shuttle between the cytoplasm and nucleus, depending on the bound nucleotide state of associated RRAGA.
- Information by UniProt
- AU041672 antibody
- FLJ13311 antibody
- GTPase interacting protein 2 antibody
All lanes : Anti-RRAGC antibody (ab168819) at 0.1 µg/ml
Lane 1 : 293T whole cell lysate
Lane 2 : HeLa whole cell lysate
Lane 3 : Jurkat whole cell lysate
Lane 4 : TCMK-1 whole cell lysate
Lane 5 : NIH3T3 whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 44 kDa
Exposure time: 3 minutes
Detection of RRAGC in Immunoprecipitates of 293T whole cell lysates (1 mg for IP, 20% of IP loaded) using ab168819 at 6 µg/mg lysate for IP (Lane 1) and at 1 µg/ml for subsequent Western blot detection.
Detection: Chemiluminescence with an exposure time of 10 seconds.
ab168819 has not yet been referenced specifically in any publications.