Key features and details
- Rabbit polyclonal to RRAGC
- Suitable for: IP, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-RRAGC antibody
See all RRAGC primary antibodies
DescriptionRabbit polyclonal to RRAGC
Tested applicationsSuitable for: IP, WBmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Sheep, Guinea pig, Cow, Dog, Pig, Orangutan
Synthetic peptide within Human RRAGC aa 1-50 (N terminal). The exact sequence is proprietary.
Database link: NP_071440.1
- HeLa, 293T, Jurkat, mouse TCMK-1 and mouse NIH3T3 cell lysates. 293T cells.
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We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab187705 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at 2-10 µg/mg of lysate.|
|WB||1/1000 - 1/5000. Predicted molecular weight: 44 kDa.|
FunctionHas guanine nucleotide-binding activity but weak intrinsic GTPase activity. Probably required for the amino acid-induced relocalization of mTORC1 to the lysosomes and its subsequent activation by the GTPase RHEB. This is a crucial step in the activation of the TOR signaling cascade by amino acids.
Sequence similaritiesBelongs to the GTR/RAG GTP-binding protein family.
Cellular localizationCytoplasm. Nucleus. Lysosome. Predominantly cytoplasmic. May shuttle between the cytoplasm and nucleus, depending on the bound nucleotide state of associated RRAGA.
- Information by UniProt
- AU041672 antibody
- FLJ13311 antibody
- GTPase interacting protein 2 antibody
Lane 1: Immunoprecipitation from 293T whole cell lysate (0.5 - 1 mg for IP, 20% of IP loaded), using ab187705 at 6 µg per reaction.
Lane 2: Immunoprecipitation from 293T whole cell lysate (0.5 - 1 mg for IP, 20% of IP loaded), using Control IgG.
Subsequent Western blot detection used ab187705 at 1 µg/ml.
All lanes : Anti-RRAGC antibody (ab187705) at 0.4 µg/ml
Lane 1 : HeLa cell lysate
Lane 2 : 293T cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : Mouse TCMK-1 cell lysate
Lane 5 : Mouse NIH3T3 cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 44 kDa
Exposure time: 30 seconds
ab187705 has not yet been referenced specifically in any publications.