Overview

  • Product name

  • Description

    Rabbit polyclonal to RRAGC
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IP, WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Sheep, Guinea pig, Cow, Dog, Pig, Orangutan
  • Immunogen

    Synthetic peptide within Human RRAGC aa 1-50 (N terminal). The exact sequence is proprietary.
    Database link: NP_071440.1

  • Positive control

    • HeLa, 293T, Jurkat, mouse TCMK-1 and mouse NIH3T3 cell lysates. 293T cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab187705 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at 2-10 µg/mg of lysate.
WB 1/1000 - 1/5000. Predicted molecular weight: 44 kDa.

Target

  • Function

    Has guanine nucleotide-binding activity but weak intrinsic GTPase activity. Probably required for the amino acid-induced relocalization of mTORC1 to the lysosomes and its subsequent activation by the GTPase RHEB. This is a crucial step in the activation of the TOR signaling cascade by amino acids.
  • Sequence similarities

    Belongs to the GTR/RAG GTP-binding protein family.
  • Cellular localization

    Cytoplasm. Nucleus. Lysosome. Predominantly cytoplasmic. May shuttle between the cytoplasm and nucleus, depending on the bound nucleotide state of associated RRAGA.
  • Information by UniProt
  • Database links

  • Alternative names

    • AU041672 antibody
    • FLJ13311 antibody
    • GTPase interacting protein 2 antibody
    • GTPase-interacting protein 2 antibody
    • GTR2 antibody
    • MGC47404 antibody
    • OTTHUMP00000000548 antibody
    • Rag C antibody
    • Rag C protein antibody
    • RAGC antibody
    • Ras related GTP binding C antibody
    • Ras-related GTP-binding protein C antibody
    • RP23 29H22.4 antibody
    • RRAGC antibody
    • RRAGC_HUMAN antibody
    • TIB929 antibody
    • YGR163W antibody
    see all

Images

  • Lane 1: Immunoprecipitation from 293T whole cell lysate (0.5 - 1 mg for IP, 20% of IP loaded), using ab187705 at 6 µg per reaction.
    Lane 2: Immunoprecipitation from 293T whole cell lysate (0.5 - 1 mg for IP, 20% of IP loaded), using Control IgG.
    Subsequent Western blot detection used ab187705 at 1 µg/ml.

  • All lanes : Anti-RRAGC antibody (ab187705) at 0.4 µg/ml

    Lane 1 : HeLa cell lysate
    Lane 2 : 293T cell lysate
    Lane 3 : Jurkat cell lysate
    Lane 4 : Mouse TCMK-1 cell lysate
    Lane 5 : Mouse NIH3T3 cell lysate

    Lysates/proteins at 50 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 44 kDa


    Exposure time: 30 seconds

References

ab187705 has not yet been referenced specifically in any publications.

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