Key features and details
- Rabbit polyclonal to RRAGC
- Suitable for: IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-RRAGC antibody
See all RRAGC primary antibodies
DescriptionRabbit polyclonal to RRAGC
Tested applicationsSuitable for: IPmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Sheep, Horse, Cow, Cat, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Giant Panda
Synthetic peptide within Human RRAGC aa 349-399. The exact sequence is proprietary. (NP_071440.1).
Database link: Q9HB90
- IP: HEK-293T whole cell lysate.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab226199 was affinity purified using an epitope specific to RRAGC immobilized on solid support.
Our Abpromise guarantee covers the use of ab226199 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at 2-10 µg/mg of lysate.|
FunctionHas guanine nucleotide-binding activity but weak intrinsic GTPase activity. Probably required for the amino acid-induced relocalization of mTORC1 to the lysosomes and its subsequent activation by the GTPase RHEB. This is a crucial step in the activation of the TOR signaling cascade by amino acids.
Sequence similaritiesBelongs to the GTR/RAG GTP-binding protein family.
Cellular localizationCytoplasm. Nucleus. Lysosome. Predominantly cytoplasmic. May shuttle between the cytoplasm and nucleus, depending on the bound nucleotide state of associated RRAGA.
- Information by UniProt
- AU041672 antibody
- FLJ13311 antibody
- GTPase interacting protein 2 antibody
RRAGC was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (20% of IP loaded) with ab226199 at 6 µg per reaction. Western blot was performed from the immunoprecipitate using ab226199 at 1 µg/ml.
Lane 1: ab226199 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG IP in HEK-293T whole cell lysate.
Detection: Chemiluminescence with exposure time of 3 minutes.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab226199 has not yet been referenced specifically in any publications.